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- W1783676257 abstract "In acidic media, ibuprofen substantially enhanced the weak chemiluminescence (CL) produced by sodium sulfite and potassium permanganate. The increased signals were linearly correlated with ibuprofen concentrations ranging from 1.2 × 10(-3) to 4.8 μM, with a detection limit of 4.8 × 10(-4) μM. Two ultrafiltration (UF) membranes were used to construct a unit for trapping 0.15 and 0.75 μM human serum albumin (HSA) and coupled online with the CL system. At low HSA concentrations, the numbers of bound molecules per binding site were calculated to be 0.9 for Sudlow site I and 6.2 for Sudlow site II. The association constants on these binding sites were 5.9 × 10(5) and 3.4 × 10(4) M(-1), respectively. Our CL-UF protocol presents a rapid and sensitive method for studies on drug-protein interaction." @default.
- W1783676257 created "2016-06-24" @default.
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- W1783676257 date "2012-12-18" @default.
- W1783676257 modified "2023-10-16" @default.
- W1783676257 title "A method of chemiluminescence coupled with ultrafiltration for investigating the interaction between ibuprofen and human serum albumin" @default.
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- W1783676257 doi "https://doi.org/10.1002/bio.2465" @default.
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