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- W178432834 abstract "This chapter describes two vectors used to introduce and express foreign genes in Spiroplasma citri. One is the replicative form of spiroplasmavirus SpV1 of S. citri, and the other is a plasmid whose replication is mediated by the chromosomal replication origin (oriC) of S. citri. This chapter also describes a protocol for efficient electrotransformation of S. citri. The use of recombinant DNA technology to study expression of mollicute genes by cloning in Escherichia coli has been limited because many mollicutes use the codon UGA to encode for tryptophan. Cloning in a spiroplasma host overcome these difficulties and help to investigate gene expression and the function of the polypeptides they encode. Transfer of genetic material into mollicutes also provides a way to complement mutations. Electroporation refers to the process of subjecting living cells to a rapidly changing, high-strength electric field, thereby producing transient pores in their outer membranes. This process has been used to introduce DNA into eukaryotic and prokaryotic cells." @default.
- W178432834 created "2016-06-24" @default.
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- W178432834 date "1995-01-01" @default.
- W178432834 modified "2023-09-27" @default.
- W178432834 title "PLASMID AND VIRAL VECTORS FOR GENE CLONING AND EXPRESSION IN SPIROPLASMA CITRI" @default.
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- W178432834 doi "https://doi.org/10.1016/b978-012583805-4/50017-0" @default.
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