FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1784660818> ?p ?o ?g. }

• W1784660818 endingPage "3901" @default.
• W1784660818 startingPage "3890" @default.
• W1784660818 abstract "ABSTRACT The intraerythrocytic apicomplexan Babesia microti , the primary causative agent of human babesiosis, is a major public health concern in the United States and elsewhere. Apicomplexans utilize a multiprotein complex that includes a type I membrane protein called apical membrane antigen 1 (AMA1) to invade host cells. We have isolated the full-length B. microti AMA1 (BmAMA1) gene and determined its nucleotide sequence, as well as the amino acid sequence of the AMA1 protein. This protein contains an N-terminal signal sequence, an extracellular region, a transmembrane region, and a short conserved cytoplasmic tail. It shows the same domain organization as the AMA1 orthologs from piroplasm, coccidian, and haemosporidian apicomplexans but differs from all other currently known piroplasmida, including other Babesia and Theileria species, in lacking two conserved cysteines in highly variable domain III of the extracellular region. Minimal polymorphism was detected in BmAMA1 gene sequences of parasite isolates from six babesiosis patients from Nantucket. Immunofluorescence microscopy studies showed that BmAMA1 is localized on the cell surface and cytoplasm near the apical end of the parasite. Native BmAMA1 from parasite lysate and refolded recombinant BmAMA1 (rBmAMA1) expressed in Escherichia coli reacted with a mouse anti-BmAMA1 antibody using Western blotting. In vitro binding studies showed that both native BmAMA1 and rBmAMA1 bind to human red blood cells (RBCs). This binding is trypsin and chymotrypsin treatment sensitive but neuraminidase independent. Incubation of B. microti parasites in human RBCs with a mouse anti-BmAMA1 antibody inhibited parasite growth by 80% in a 24-h assay. Based on its antigenically conserved nature and potential role in RBC invasion, BmAMA1 should be evaluated as a vaccine candidate." @default.
• W1784660818 created "2016-06-24" @default.
• W1784660818 creator A5008958885 @default.
• W1784660818 creator A5024629664 @default.
• W1784660818 creator A5026932356 @default.
• W1784660818 creator A5055168891 @default.
• W1784660818 creator A5062031724 @default.
• W1784660818 creator A5062041588 @default.
• W1784660818 creator A5064369996 @default.
• W1784660818 creator A5078743899 @default.
• W1784660818 creator A5078847584 @default.
• W1784660818 creator A5086085758 @default.
• W1784660818 date "2015-10-01" @default.
• W1784660818 modified "2023-10-18" @default.
• W1784660818 title "Expression, Purification, and Biological Characterization of Babesia microti Apical Membrane Antigen 1" @default.
• W1784660818 cites W1543944205 @default.
• W1784660818 cites W1558460014 @default.
• W1784660818 cites W1965804686 @default.
• W1784660818 cites W1968378375 @default.
• W1784660818 cites W1968779685 @default.
• W1784660818 cites W1970047032 @default.
• W1784660818 cites W1972736568 @default.
• W1784660818 cites W1978578424 @default.
• W1784660818 cites W1983260712 @default.
• W1784660818 cites W1983910626 @default.
• W1784660818 cites W1985204564 @default.
• W1784660818 cites W1986576600 @default.
• W1784660818 cites W1986862574 @default.
• W1784660818 cites W1987240336 @default.
• W1784660818 cites W1990372674 @default.
• W1784660818 cites W2008039338 @default.
• W1784660818 cites W2011065921 @default.
• W1784660818 cites W2016085134 @default.
• W1784660818 cites W2021282986 @default.
• W1784660818 cites W2021323752 @default.
• W1784660818 cites W2024127953 @default.
• W1784660818 cites W2026483075 @default.
• W1784660818 cites W2030383801 @default.
• W1784660818 cites W2033980450 @default.
• W1784660818 cites W2037606550 @default.
• W1784660818 cites W2042323803 @default.
• W1784660818 cites W2045586186 @default.
• W1784660818 cites W2052664734 @default.
• W1784660818 cites W2054384493 @default.
• W1784660818 cites W2055780930 @default.
• W1784660818 cites W2067926806 @default.
• W1784660818 cites W2073179858 @default.
• W1784660818 cites W2073241690 @default.
• W1784660818 cites W2080018424 @default.
• W1784660818 cites W2083126580 @default.
• W1784660818 cites W2083692164 @default.
• W1784660818 cites W2085750319 @default.
• W1784660818 cites W2085835971 @default.
• W1784660818 cites W2088608170 @default.
• W1784660818 cites W2088970729 @default.
• W1784660818 cites W2090387688 @default.
• W1784660818 cites W2093550330 @default.
• W1784660818 cites W2107794793 @default.
• W1784660818 cites W2115924349 @default.
• W1784660818 cites W2117235669 @default.
• W1784660818 cites W2117929568 @default.
• W1784660818 cites W2127227156 @default.
• W1784660818 cites W2133312664 @default.
• W1784660818 cites W2133627748 @default.
• W1784660818 cites W2137510730 @default.
• W1784660818 cites W2139849462 @default.
• W1784660818 cites W2147295137 @default.
• W1784660818 cites W2147595745 @default.
• W1784660818 cites W2148570035 @default.
• W1784660818 cites W2149842796 @default.
• W1784660818 cites W2161366128 @default.
• W1784660818 cites W2162518989 @default.
• W1784660818 cites W2164762368 @default.
• W1784660818 cites W2167100261 @default.
• W1784660818 cites W2167507121 @default.
• W1784660818 cites W2170848108 @default.
• W1784660818 cites W2171457815 @default.
• W1784660818 cites W2188951095 @default.
• W1784660818 cites W4249259347 @default.
• W1784660818 cites W4253746234 @default.
• W1784660818 doi "https://doi.org/10.1128/iai.00168-15" @default.
• W1784660818 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4567623" @default.
• W1784660818 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26195550" @default.
• W1784660818 hasPublicationYear "2015" @default.
• W1784660818 type Work @default.
• W1784660818 sameAs 1784660818 @default.
• W1784660818 citedByCount "24" @default.
• W1784660818 countsByYear W17846608182016 @default.
• W1784660818 countsByYear W17846608182017 @default.
• W1784660818 countsByYear W17846608182018 @default.
• W1784660818 countsByYear W17846608182020 @default.
• W1784660818 countsByYear W17846608182021 @default.
• W1784660818 countsByYear W17846608182022 @default.
• W1784660818 countsByYear W17846608182023 @default.
• W1784660818 crossrefType "journal-article" @default.
• W1784660818 hasAuthorship W1784660818A5008958885 @default.
• W1784660818 hasAuthorship W1784660818A5024629664 @default.
• W1784660818 hasAuthorship W1784660818A5026932356 @default.

• next