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- W17849380 abstract "Publisher Summary This chapter discusses various methods for isolation of pregnanediol and pregnanetriol. The first attempt at a quantitative determination of urinary pregnanediol was based on technique for isolating the sodium pregnanediol glucuronoside complex. The complex was extracted from urine with butanol that was washed with strong alkali solution. The residue left after evaporation of the butanol was then twice precipitated from aqueous solution by the addition of acetone. Finally, the precipitate was dried and weighed. With subsequent methods, however, it has been possible to eliminate the unwanted chromogens resulting from acid hydrolysis. As enzyme hydrolysis is more laborious and because aberrant enzyme inhibitors may occur in urine, modern methods have favored acid hydrolysis wherever possible. Specificity is the degree to which only the material to be assayed is measured in the final determination. There is no known specific reaction for pregnanediol or pregnanetriol. The specificity of any assay method, therefore, depends to a large extent on how completely the steroid can be separated from the other constituents of urine and from the substances employed in their isolation." @default.
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- W17849380 date "1962-01-01" @default.
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- W17849380 title "Pregnanediol and Pregnanetriol" @default.
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- W17849380 doi "https://doi.org/10.1016/b978-1-4832-3033-7.50010-6" @default.
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