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• W1785231567 endingPage "336" @default.
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• W1785231567 abstract "Donnellan, J. Edward, Jr. (U.S. Army Natick Laboratories, Natick, Mass.), Ella H. Nags, and Hillel S. Levinson . Chemically defined, synthetic media for sporulation and for germination and growth of Bacillus subtilis . J. Bacteriol. 87: 332–336. 1964.—From 90 to 130 mg (dry weight) of spores (about 1% dark forms) were obtained (per liter) from a chemically defined, synthetic medium, with a two-phase (polyethylene glycol-potassium phosphate) harvest procedure. Optimal sporulation occurred when glucose and glutamic acid were at a concentration of 10 m m in the medium. Ca ++ and Mn ++ were required for sporulation. Heat resistance, dipicolinic acid content, and properties of germination and postgerminative development of spores grown in different concentrations of Ca ++ were investigated. Heat shock did not increase germination of spores derived from the synthetic medium. A synthetic medium, in which spore germination, emergence, and first cell division approached synchrony, was devised." @default.
• W1785231567 created "2016-06-24" @default.
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• W1785231567 date "1964-02-01" @default.
• W1785231567 modified "2023-10-16" @default.
• W1785231567 title "CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF <i>BACILLUS SUBTILIS</i>" @default.
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• W1785231567 doi "https://doi.org/10.1128/jb.87.2.332-336.1964" @default.
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