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- W1785502079 abstract "A fast, sensitive, and selective method for the determination of histamine in human urine samples by ultrahigh pressure liquid chromatography (LC) with fluorescence and mass spectrometry (MS) detection is investigated. A fluorescent reagent, 4-(1-pyrene) butyric acid N-hydroxysuccinimide ester was conjugated to the primary and secondary amino moieties of histamine. The structure of dipyrene-labeled histamine in human urine was determined by quadrupole time-of-flight MS with electospray ionization interface. The determination of the dipyrene derivative of histamine in urine samples was achieved within 3.9 min on an ultrahigh pressure LC Eclipse Zorbax XDB-C18 column with 1.8 μm particle diameter. In this work, histamine separation was achieved significantly faster (3.9 min) with improved detection limit (signal-to-noise = 3) of 0.04 nM than 19.5 min with a detection limit of 0.183 nM as reported in a previous method." @default.
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- W1785502079 date "2012-05-01" @default.
- W1785502079 modified "2023-09-23" @default.
- W1785502079 title "Histamine determination in human urine using sub-2 μm C18 column with fluorescence and mass spectrometric detection" @default.
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- W1785502079 doi "https://doi.org/10.1002/jssc.201101045" @default.
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