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• W1785663566 startingPage "1033" @default.
• W1785663566 abstract "DNA sequences from the Candida utilis genome which, when cloned into a yeast integration plasmid (YIp5), confer on YIp5 the ability to replicate autonomously in Saccharomyces cerevisiae are described. Several recombinant plasmids which transform S. cerevisiae YNN27 to Ura3+ with an efficiency of 2 X 10(3) transformants per microgram of DNA were obtained. One of the recombinant plasmids, pHMR22 (6.6 kilobases) contains ars (autonomous replication sequence), which is homologous with two different DNA fragments of the C. utilis genome but has no detectable homology to total DNA from Candida albicans, Pachysolen tannophilus, or S. cerevisiae. Restriction and subcloning analyses of pHMR22 showed that Sau3A destroys the functions of cloned ars whereas there are no BamHI, PstI, SalI, HindIII, EcoRI, or PvuII sites in the region of ars which is required for its functional integrity. Thus, pHMR22 appears to be a useful vector for cloning desired genes in S. cerevisiae." @default.
• W1785663566 created "2016-06-24" @default.
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• W1785663566 date "1983-06-01" @default.
• W1785663566 modified "2023-09-25" @default.
• W1785663566 title "Construction of a new yeast cloning vector containing autonomous replication sequences from Candida utilis" @default.
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• W1785663566 doi "https://doi.org/10.1128/jb.154.3.1033-1039.1983" @default.
• W1785663566 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/217572" @default.
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