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• W1785856027 startingPage "5423" @default.
• W1785856027 abstract "Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed from aerobic to anaerobic and that this induction was independent of Fnr but dependent on regulators AppY and ArcA. Furthermore, we found that the transcription of the hya operon was not regulated by the cyclic AMP-cyclic AMP receptor protein complex. Investigation of the effects of different anaerobic growth conditions on the expression of the hya operon showed that expression was induced by formate and repressed by nitrate. Formate induction was not mediated by the fhlA gene product, and nitrate repression was not mediated by the narL gene product. We found a high level of anaerobic expression of the hya operon in glucose medium supplemented with formate and in glycerol medium supplemented with fumarate, suggesting that hydrogenase isoenzyme 1 has a function during both fermentative growth and anaerobic respiration." @default.
• W1785856027 created "2016-06-24" @default.
• W1785856027 creator A5036408729 @default.
• W1785856027 creator A5065875262 @default.
• W1785856027 date "1994-09-01" @default.
• W1785856027 modified "2023-10-17" @default.
• W1785856027 title "Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli" @default.
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• W1785856027 doi "https://doi.org/10.1128/jb.176.17.5423-5428.1994" @default.
• W1785856027 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/196730" @default.
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