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• W1787979257 abstract "Summary Bacterial cytokinesis is driven by the septal ring apparatus, the assembly of which in Escherichia coli is directed to mid‐cell by the Min system. Despite suffering aberrant divisions at the poles, cells lacking the minCDE operon (Min – ) have an almost normal growth rate. We developed a generally applicable screening method for synthetic lethality in E. coli , and used it to select for transposon mutations ( slm ) that are s ynthetically l ethal (or sick) in combination with Δ m inCDE . One of the slm insertions mapped to envC ( yibP ), proposed to encode a lysostaphin‐like, metallo‐endopeptidase that is exported to the periplasm by the general secretory (Sec) pathway. Min – EnvC – cells showed a severe division defect, supporting a role for EnvC in septal ring function. Accordingly, we show that an EnvC–green fluorescent protein fusion, when directed to the periplasm via the twin‐arginine export system, is both functional and part of the septal ring apparatus. Using an in‐gel assay, we also present evidence that EnvC possesses murein hydrolytic activity. Our results suggest that EnvC plays a direct role in septal murein cleavage to allow outer membrane constriction and daughter cell separation. By uncovering genetic interactions, the synthetic lethal screen described here provides an attractive new tool for studying gene function in E. coli ." @default.
• W1787979257 created "2016-06-24" @default.
• W1787979257 creator A5020999516 @default.
• W1787979257 creator A5070340434 @default.
• W1787979257 date "2004-04-28" @default.
• W1787979257 modified "2023-10-18" @default.
• W1787979257 title "Screening for synthetic lethal mutants in <i>Escherichia coli</i> and identification of EnvC (YibP) as a periplasmic septal ring factor with murein hydrolase activity" @default.
• W1787979257 cites W1487600762 @default.
• W1787979257 cites W1530539563 @default.
• W1787979257 cites W1532029656 @default.
• W1787979257 cites W1549355483 @default.
• W1787979257 cites W1671789228 @default.
• W1787979257 cites W1682676750 @default.
• W1787979257 cites W1714832738 @default.
• W1787979257 cites W1853561639 @default.
• W1787979257 cites W1919928164 @default.
• W1787979257 cites W1964601681 @default.
• W1787979257 cites W1970793802 @default.
• W1787979257 cites W1977050371 @default.
• W1787979257 cites W1979622043 @default.
• W1787979257 cites W198085179 @default.
• W1787979257 cites W1983516501 @default.
• W1787979257 cites W1989242934 @default.
• W1787979257 cites W1990033090 @default.
• W1787979257 cites W2000214816 @default.
• W1787979257 cites W2008228685 @default.
• W1787979257 cites W2008433064 @default.
• W1787979257 cites W2009415450 @default.
• W1787979257 cites W2009574796 @default.
• W1787979257 cites W2023890960 @default.
• W1787979257 cites W2034954676 @default.
• W1787979257 cites W2041417051 @default.
• W1787979257 cites W2042814467 @default.
• W1787979257 cites W2043539143 @default.
• W1787979257 cites W2049153173 @default.
• W1787979257 cites W2050088265 @default.
• W1787979257 cites W2050787463 @default.
• W1787979257 cites W2052800057 @default.
• W1787979257 cites W2067477145 @default.
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• W1787979257 cites W2070254699 @default.
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• W1787979257 cites W2077176951 @default.
• W1787979257 cites W2081426177 @default.
• W1787979257 cites W2085748469 @default.
• W1787979257 cites W2086076872 @default.
• W1787979257 cites W2089016671 @default.
• W1787979257 cites W2095079168 @default.
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• W1787979257 cites W2100809095 @default.
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• W1787979257 cites W2110743190 @default.
• W1787979257 cites W2111198021 @default.
• W1787979257 cites W2112704964 @default.
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• W1787979257 cites W2142104231 @default.
• W1787979257 cites W2142779706 @default.
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• W1787979257 cites W2156910074 @default.
• W1787979257 cites W2157915163 @default.
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• W1787979257 doi "https://doi.org/10.1111/j.1365-2958.2004.04063.x" @default.
• W1787979257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4428336" @default.
• W1787979257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15165230" @default.
• W1787979257 hasPublicationYear "2004" @default.
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