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- W1792161851 abstract "This work presents a new strategy of the combination of surface plasmon resonance (SPR) and electrochemical study for real-time evaluation of live cancer cells treated with daunorubicin (DNR) at the interface of the SPR chip and living cancer cells. The observations demonstrate that the SPR signal changes could be closely related to the morphology and mass changes of adsorbed cancer cells and the variation of the refractive index of the medium solution. The results of light microscopy images and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide studies also illustrate the release or desorption of HepG2 cancer cells, which were due to their apoptosis after treatment with DNR. It is evident that the extracellular concentration of DNR residue can be readily determined through electrochemical measurements. The decreases in the magnitudes of SPR signals were linearly related to cell survival rates, and the combination of SPR with electrochemical study could be utilized to evaluate the potential therapeutic efficiency of bioactive agents to cells. Thus, this label-free, real-time SPR–electrochemical detection technique has great promise in bioanalysis or monitoring of relevant treatment processes in clinical applications." @default.
- W1792161851 created "2016-06-24" @default.
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- W1792161851 date "2015-10-29" @default.
- W1792161851 modified "2023-10-16" @default.
- W1792161851 title "Real-Time Evaluation of Live Cancer Cells by an <i>in Situ</i> Surface Plasmon Resonance and Electrochemical Study" @default.
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- W1792161851 doi "https://doi.org/10.1021/acsami.5b08066" @default.
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