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- W1793358268 abstract "Bacteriophages, also known as phages, are viruses that are ubiquitous and survive and replicate within the host of the bacterial cell, Mycobacterium smegmatis. They are considered one of the most abundant organisms on earth (1031). Structurally, they are 100-200nm in size and consist of a protein encapsulated head that contains DNA or RNA, a tail sheath and tail fibers. This research consisted of examining phage infection, by re-isolating a novel phage, Tango. Tango was originally isolated genetically in 2013 by a previous ISBT student, Anna Maccarrone. The phage was sent to genetic sequencing but two phages were discovered, instead of one, in clusters B and C. The genetic annotation was not able to be analyzed due to phage mutation and impurity. During spring 2015, this phage was re-visited following laboratory protocol specific to this experiment as well as incorporating my own ideas with the goal of re-isolating, purifying and extracting the genetic material using DNASE I and RNASE A. Genetic material extraction would also include electrophoresis, as well as spectrophotometric studies and future DNA sequencing. I N T R O D U C T I O N Mycobacterium smegmatis, strain mc2 155, is a non-pathogenic bacterium that grows rapidly within 72 hours and is about 3.0 to 5.0 μm in diameter. It acts as a host for bacteriophages, which are viruses that are ubiquitous and include as many as 1031different organisms since there are considered the most abundant organism on earth. The phages have the ability to infect and replicate within the host and will eventually lyse the bacteria. Temperate phages, especially lambda phages have the ability to enter into a dormant phase, where the genes are not expressed until it reverts to the lytic phase. During the lytic phase, the phage will inject its genetic material into its host and replicate itself until it consumes most to all of the bacteria, thus forming a plaque as evidence." @default.
- W1793358268 created "2016-06-24" @default.
- W1793358268 creator A5068947211 @default.
- W1793358268 date "2015-01-01" @default.
- W1793358268 modified "2023-09-27" @default.
- W1793358268 title "Examining Phage Infection Utilizing Mycobacterium Smegmatis" @default.
- W1793358268 hasPublicationYear "2015" @default.
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