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• W1794693722 endingPage "602" @default.
• W1794693722 startingPage "590" @default.
• W1794693722 abstract "The MtrCDE multidrug pump, from Neisseria gonorrhoeae, is assembled from the inner and outer membrane proteins MtrD and MtrE, which are connected by the periplasmic membrane fusion protein MtrC. Although it is clear that MtrD delivers drugs to the channel of MtrE, it remains unclear how drug delivery and channel opening are connected. We used a vancomycin sensitivity assay to test for opening of the MtrE channel. Cells expressing MtrE or MtrE-E434K were insensitive to vancomycin; but became moderately and highly sensitive to vancomycin respectively, when coexpressed with MtrC, suggesting that the MtrE channel opening requires MtrC binding and is energy-independent. Cells expressing wild-type MtrD, in an MtrCE background, were vancomycin-insensitive, but moderately sensitive in an MtrCE-E434K background. The mutation of residues involved in proton translocation inactivated MtrD and abolished drug efflux, rendered both MtrE and MtrE-E434K vancomycin-insensitive; imply that the pump–component interactions are preserved, and that the complex is stable in the absence of proton flux, thus sealing the open end of MtrE. Following the energy-dependent dissociation of the tripartite complex, the MtrE channel is able to reseal, while MtrE-E434K is unable to do so, resulting in the vancomycin-sensitive phenotype. Thus, our findings suggest that opening of the OMP via interaction with the MFP is energy-independent, while both drug export and complex dissociation require active proton flux." @default.
• W1794693722 created "2016-06-24" @default.
• W1794693722 creator A5026488732 @default.
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• W1794693722 date "2013-04-09" @default.
• W1794693722 modified "2023-10-03" @default.
• W1794693722 title "Tripartite efflux pumps: energy is required for dissociation, but not assembly or opening of the outer membrane channel of the pump" @default.
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• W1794693722 doi "https://doi.org/10.1111/mmi.12211" @default.
• W1794693722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3664412" @default.
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