FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W179648318> ?p ?o ?g. }

• W179648318 endingPage "163" @default.
• W179648318 startingPage "159" @default.
• W179648318 abstract "A bacterial expression system has been engineered for human glutamine synthetase (EC 6.3.1.2) that produces approximately 60 mg of enzyme (20% of the bacterial soluble protein) and yields approx. 8 mg of purified enzyme per litre of culture. The recombinant enzyme was purified 5-fold to apparent homogeneity and characterized. It has a subunit molecular mass of approx. 45000 Da. The Vmax value obtained using a radioactive assay with ammonia and l-[G-3H]glutamic acid as substrates was 15.9 micromol/min per mg, 40% higher than that obtained in the colorimetric assay (9.9 micromol/min per mg) with hydroxylamine replacing ammonia as a substrate. Km values for glutamate were 3.0 mM and 3.5 mM, and for ATP they were 2.0 mM and 2. 9 mM for the radioactive and spectrophotometric assays respectively. The Km for ammonia in the radioactive assay was 0.15 mM. The midpoint of thermal inactivation was 49.7 degrees C. Hydroxylamine, Mg(II) and Mg(II)-ATP stabilized the enzyme against thermal inactivation, whereas ATP promoted inactivation. The pure enzyme is stable for several months in storage and provides a source for additional studies, including X-ray crystallography." @default.
• W179648318 created "2016-06-24" @default.
• W179648318 creator A5015152859 @default.
• W179648318 creator A5020960658 @default.
• W179648318 creator A5036549445 @default.
• W179648318 creator A5058865567 @default.
• W179648318 creator A5067998476 @default.
• W179648318 creator A5072857023 @default.
• W179648318 date "1997-11-15" @default.
• W179648318 modified "2023-10-18" @default.
• W179648318 title "Expression, purification, and characterization of recombinant human glutamine synthetase" @default.
• W179648318 cites W1506041082 @default.
• W179648318 cites W1516703335 @default.
• W179648318 cites W1550024277 @default.
• W179648318 cites W1550736140 @default.
• W179648318 cites W1555967769 @default.
• W179648318 cites W1713124107 @default.
• W179648318 cites W1785497696 @default.
• W179648318 cites W1968807182 @default.
• W179648318 cites W1982545384 @default.
• W179648318 cites W1984089779 @default.
• W179648318 cites W1987705217 @default.
• W179648318 cites W1992630712 @default.
• W179648318 cites W1994542508 @default.
• W179648318 cites W2002575340 @default.
• W179648318 cites W2004380296 @default.
• W179648318 cites W2018101699 @default.
• W179648318 cites W2018217993 @default.
• W179648318 cites W2027951690 @default.
• W179648318 cites W2033651371 @default.
• W179648318 cites W2033815236 @default.
• W179648318 cites W2044240340 @default.
• W179648318 cites W2045209911 @default.
• W179648318 cites W2058868228 @default.
• W179648318 cites W2061538287 @default.
• W179648318 cites W2075902432 @default.
• W179648318 cites W2076835106 @default.
• W179648318 cites W2122504167 @default.
• W179648318 cites W2128635872 @default.
• W179648318 cites W2133263245 @default.
• W179648318 cites W2138270253 @default.
• W179648318 cites W2147409100 @default.
• W179648318 cites W2171685249 @default.
• W179648318 cites W2394894015 @default.
• W179648318 cites W2398467441 @default.
• W179648318 cites W2467497552 @default.
• W179648318 cites W48970548 @default.
• W179648318 doi "https://doi.org/10.1042/bj3280159" @default.
• W179648318 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1218900" @default.
• W179648318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9359847" @default.
• W179648318 hasPublicationYear "1997" @default.
• W179648318 type Work @default.
• W179648318 sameAs 179648318 @default.
• W179648318 citedByCount "41" @default.
• W179648318 countsByYear W1796483182012 @default.
• W179648318 countsByYear W1796483182013 @default.
• W179648318 countsByYear W1796483182014 @default.
• W179648318 countsByYear W1796483182015 @default.
• W179648318 countsByYear W1796483182016 @default.
• W179648318 countsByYear W1796483182017 @default.
• W179648318 countsByYear W1796483182018 @default.
• W179648318 countsByYear W1796483182019 @default.
• W179648318 countsByYear W1796483182020 @default.
• W179648318 countsByYear W1796483182021 @default.
• W179648318 countsByYear W1796483182022 @default.
• W179648318 countsByYear W1796483182023 @default.
• W179648318 crossrefType "journal-article" @default.
• W179648318 hasAuthorship W179648318A5015152859 @default.
• W179648318 hasAuthorship W179648318A5020960658 @default.
• W179648318 hasAuthorship W179648318A5036549445 @default.
• W179648318 hasAuthorship W179648318A5058865567 @default.
• W179648318 hasAuthorship W179648318A5067998476 @default.
• W179648318 hasAuthorship W179648318A5072857023 @default.
• W179648318 hasBestOaLocation W1796483182 @default.
• W179648318 hasConcept C104292427 @default.
• W179648318 hasConcept C104317684 @default.
• W179648318 hasConcept C13965031 @default.
• W179648318 hasConcept C153911025 @default.
• W179648318 hasConcept C181199279 @default.
• W179648318 hasConcept C185592680 @default.
• W179648318 hasConcept C18903297 @default.
• W179648318 hasConcept C198684833 @default.
• W179648318 hasConcept C2775999725 @default.
• W179648318 hasConcept C2776382133 @default.
• W179648318 hasConcept C2777289219 @default.
• W179648318 hasConcept C2778740566 @default.
• W179648318 hasConcept C2779289688 @default.
• W179648318 hasConcept C2779349466 @default.
• W179648318 hasConcept C28212737 @default.
• W179648318 hasConcept C40767141 @default.
• W179648318 hasConcept C43617362 @default.
• W179648318 hasConcept C515207424 @default.
• W179648318 hasConcept C55493867 @default.
• W179648318 hasConcept C86803240 @default.
• W179648318 hasConceptScore W179648318C104292427 @default.
• W179648318 hasConceptScore W179648318C104317684 @default.
• W179648318 hasConceptScore W179648318C13965031 @default.
• W179648318 hasConceptScore W179648318C153911025 @default.

• next