Matches in SemOpenAlex for { <https://semopenalex.org/work/W17966160> ?p ?o ?g. }
- W17966160 abstract "Abstract Proper regulation of the human CD34 gene requires a combinatorial action of multiple proximal and long-range, ciselements. This report shows that, like the murine CD34 5′ untranslated region (UTR), the corresponding region of the human CD34 gene is necessary for optimal promoter activity. We localized the most critical element of this region to base pairs +48/+75. Through oligonucleotide competition and antibody supershift experiments in electrophoretic mobility shift assays, we found that this sequence contains a binding site (CCAAT box) for the transcription factor NFY (nuclear factor Y), a factor mediating cell type-specific and cell-cycle regulated expression of genes. Mutating this site led to a 5-fold decrease in CD34 promoter activity in transient transfection experiments. Interestingly, NFY binds adjacently to the earlier identified c-myb binding site. Here we show that both binding sites are important for CD34 promoter function: mutating either site alone decreased CD34 promoter-driven reporter gene activity 4-fold. We also show that the integrity of the c-myb binding site is necessary for stabilization of NFY binding to its site. Such cooperation between c-myb, which is expressed in early hematopoietic cells, and NFY, which is expressed in many cell types, might contribute to specific activation of CD34 in stem cells. The CCAAT box motif was also noted in the 5′ UTR of the murine CD34 gene, however, NFY did not bind to this region. Thus, our results indicate that the functional similarities between the human and murine CD34 5′ UTRs are achieved through different molecular mechanism(s)." @default.
- W17966160 created "2016-06-24" @default.
- W17966160 creator A5004477857 @default.
- W17966160 creator A5007253657 @default.
- W17966160 creator A5033836751 @default.
- W17966160 creator A5043093495 @default.
- W17966160 creator A5045152041 @default.
- W17966160 creator A5056687452 @default.
- W17966160 date "1999-12-01" @default.
- W17966160 modified "2023-09-28" @default.
- W17966160 title "A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene" @default.
- W17966160 cites W117186332 @default.
- W17966160 cites W1216602061 @default.
- W17966160 cites W1495242750 @default.
- W17966160 cites W1495660248 @default.
- W17966160 cites W1502074492 @default.
- W17966160 cites W1535685697 @default.
- W17966160 cites W1540456190 @default.
- W17966160 cites W1541937812 @default.
- W17966160 cites W1556415839 @default.
- W17966160 cites W1575380102 @default.
- W17966160 cites W1656415435 @default.
- W17966160 cites W170947191 @default.
- W17966160 cites W1896728310 @default.
- W17966160 cites W1968561263 @default.
- W17966160 cites W1969772407 @default.
- W17966160 cites W1978630335 @default.
- W17966160 cites W1987719171 @default.
- W17966160 cites W1994306146 @default.
- W17966160 cites W1995817356 @default.
- W17966160 cites W1999966058 @default.
- W17966160 cites W200647492 @default.
- W17966160 cites W2013545053 @default.
- W17966160 cites W2020584790 @default.
- W17966160 cites W2026168151 @default.
- W17966160 cites W2033513498 @default.
- W17966160 cites W2036553614 @default.
- W17966160 cites W2037824747 @default.
- W17966160 cites W2038183822 @default.
- W17966160 cites W2052687631 @default.
- W17966160 cites W2060017591 @default.
- W17966160 cites W2067616847 @default.
- W17966160 cites W2081267992 @default.
- W17966160 cites W2093988305 @default.
- W17966160 cites W2094838950 @default.
- W17966160 cites W2099240952 @default.
- W17966160 cites W2104808124 @default.
- W17966160 cites W2127385262 @default.
- W17966160 cites W2129382795 @default.
- W17966160 cites W2146026647 @default.
- W17966160 cites W2160074522 @default.
- W17966160 cites W2162709420 @default.
- W17966160 cites W2163584800 @default.
- W17966160 cites W2171882089 @default.
- W17966160 cites W2182786267 @default.
- W17966160 cites W2221459232 @default.
- W17966160 cites W2232014030 @default.
- W17966160 cites W2257703597 @default.
- W17966160 cites W2303930516 @default.
- W17966160 cites W4214728784 @default.
- W17966160 cites W4251845825 @default.
- W17966160 cites W4254795982 @default.
- W17966160 cites W4293247451 @default.
- W17966160 cites W73118701 @default.
- W17966160 doi "https://doi.org/10.1182/blood.v94.11.3772" @default.
- W17966160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10572091" @default.
- W17966160 hasPublicationYear "1999" @default.
- W17966160 type Work @default.
- W17966160 sameAs 17966160 @default.
- W17966160 citedByCount "34" @default.
- W17966160 countsByYear W179661602012 @default.
- W17966160 countsByYear W179661602013 @default.
- W17966160 countsByYear W179661602016 @default.
- W17966160 countsByYear W179661602017 @default.
- W17966160 countsByYear W179661602020 @default.
- W17966160 countsByYear W179661602021 @default.
- W17966160 crossrefType "journal-article" @default.
- W17966160 hasAuthorship W17966160A5004477857 @default.
- W17966160 hasAuthorship W17966160A5007253657 @default.
- W17966160 hasAuthorship W17966160A5033836751 @default.
- W17966160 hasAuthorship W17966160A5043093495 @default.
- W17966160 hasAuthorship W17966160A5045152041 @default.
- W17966160 hasAuthorship W17966160A5056687452 @default.
- W17966160 hasConcept C101762097 @default.
- W17966160 hasConcept C104317684 @default.
- W17966160 hasConcept C105580179 @default.
- W17966160 hasConcept C107824862 @default.
- W17966160 hasConcept C150194340 @default.
- W17966160 hasConcept C153911025 @default.
- W17966160 hasConcept C161733203 @default.
- W17966160 hasConcept C179716448 @default.
- W17966160 hasConcept C54355233 @default.
- W17966160 hasConcept C86339819 @default.
- W17966160 hasConcept C86803240 @default.
- W17966160 hasConcept C89604277 @default.
- W17966160 hasConceptScore W17966160C101762097 @default.
- W17966160 hasConceptScore W17966160C104317684 @default.
- W17966160 hasConceptScore W17966160C105580179 @default.
- W17966160 hasConceptScore W17966160C107824862 @default.
- W17966160 hasConceptScore W17966160C150194340 @default.