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• W1796807472 abstract "The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). The gene is transcribed into a 1250-base transcript. The expression of the flavodoxin gene was analysed and found to be regulated at the transcriptional level by the availability of iron. The PCR-amplified gene was cloned into the expression vector pTrc 99b and expressed in Escherichia coli. High concentrations of flavodoxin were found (20% of total protein). The recombinant protein was purified from the cytosolic fraction of the cells and it exhibited properties identical with those of the wild-type Anabaena flavodoxin." @default.
• W1796807472 created "2016-06-24" @default.
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• W1796807472 date "1991-11-15" @default.
• W1796807472 modified "2023-09-30" @default.
• W1796807472 title "Isolation and overexpression in <i>Escherichia coli</i> of the flavodoxin gene from <i>Anabaena</i> PCC 7119" @default.
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• W1796807472 doi "https://doi.org/10.1042/bj2800187" @default.
• W1796807472 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1130618" @default.
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