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- W179849078 abstract "Publisher Summary Budding yeast offers numerous tools and methods suitable for quantitative analysis of microtubule dynamics and function in living cells, including collections of strains bearing loss of function open reading frame (ORF deletion) mutations or expressing carboxyl terminal enhanced GFP (EGFP) fusion proteins. These resources offer tremendous potential for discovery when used with care. Regulation of microtubule dynamics requires microtubule-associating proteins (MAPs) including microtubule plus-end tracking proteins (+TIPs) and plus- and minus-end-directed motor proteins. Potential mechanisms for +TIP tracking of microtubule plus-ends include treadmilling, transport, and hitchhiking. Manual analysis of microtubule dynamics requires a careful experimental setup to ensure completeness and consistency of the data set. First, each movie must be analyzed several times and tracks must be averaged to minimize tracking bias introduced by the investigator. Then, the necessary amount of data points to estimate each descriptor of microtubule dynamics needs to be determined. Finally, movies need to be analyzed in an unbiased manner to avoid artificially enriching for a specific phenotype. This can be achieved by having different investigators preselect cells for analysis. Although automated tracking of microtubules requires a substantial initial investment to establishment of the necessary software tools, it provides several advantages over manual microtubule tracking. First, microtubules can be tracked at subpixel localization, increasing tracking precision significantly. Second intra-and interuser variability of tracking results is eliminated when using automated tracking methods. Third the amount of data that can be analyzed when using computer-aided tracking is unmatched." @default.
- W179849078 created "2016-06-24" @default.
- W179849078 creator A5041368919 @default.
- W179849078 creator A5042912246 @default.
- W179849078 creator A5082312534 @default.
- W179849078 date "2010-01-01" @default.
- W179849078 modified "2023-09-26" @default.
- W179849078 title "Analysis of Microtubules in Budding Yeast" @default.
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