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- W179910092 abstract "Three miniature swine lines, inbred for SLA haplotypes a, C/ and df and a recombinant line, haplotype £, were analyzed for possible restriction fragment length polymorphisms (RFLPs) by Southern blot hybridization with human C2 and factor B (Bf) specific probes. The search for RFLPs by using a human C2 probe failed to reveal any variants. However, a TaqI polymorphism was identified with a human Bf probe. Over lapping restriction fragments were found with C2 and Bf probes, which strongly suggests close linkage of C2 and Bf genes in swine. Two TaqI RFLP patterns were distinguished with the Bf probe. Segregation analyses indicated that the polymorphic fragments followed a Mendelian pattern of inheritance. The recombinant haplotype c[, which expresses class I genes of haplotype c and class II genes of haplotype d, was shown to produce an identical RFLP pattern, using the Bf probe, as haplotype d, but different from that of haplotype c. This indicates that there is a close association between Bf and class II genes and that the recombination event leading to the £ haplotype must have occurred between the class'II-Bf and class I genes. Although these data do not show conclusively the location of the C2-Bf genes, it is hypothesized that swine C2-Bf is located between the class II and class I genes, as has been demonstrated in mouse and man." @default.
- W179910092 created "2016-06-24" @default.
- W179910092 creator A5021337832 @default.
- W179910092 date "2018-08-13" @default.
- W179910092 modified "2023-09-26" @default.
- W179910092 title "Analysis and characterization of the swine major histocompatibility complex" @default.
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