Matches in SemOpenAlex for { <https://semopenalex.org/work/W1799231693> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W1799231693 endingPage "168" @default.
- W1799231693 startingPage "147" @default.
- W1799231693 abstract "GENOMICS SEQUENCING OF GENOMES As mentioned in Chapter 3, we now know the complete nucleotide sequences of genomes of many organisms. The availability of this large amount of data at an unprecedented scale now allows us, and indeed forces us, to think “globally,” that is, on the scale of whole organisms, or even an assemblage of organisms, rather than of individual genes and enzymes. Here we describe very briefly how the genome sequences are determined. Genome sequencing of viruses began in the late 1970s. The basic technique involved, the random sequencing of fragments by the Sanger dideoxy termination method, was proposed and applied successfully by Fred Sanger and associates to the complete sequencing of bacteriophage DNAs, notably that of phage λ in 1980 (see Figure 4.1 for the principle of the random shotgun method). Historically, the first attempt to obtain a complete genome sequence of a cellular organism was geared toward Escherichia coli , the best studied organism outside of humans. This project started in 1989 and used a “directed” approach. Because a fairly detailed genetic map of E. coli was available thanks to the efforts of bacterial geneticists, it was possible to first produce a set of λ-based clones, each containing up to 20 kb DNA, with overlapping ends. The sequencing from here on represented the shotgun phase. The inserted segments in the λ vector were then randomly cut into much smaller fragments of a few kilobases, they were cloned into an M13 vector and were sequenced (for sequencing reactions, see Box 4.1), and the sequences were assembled by looking for overlaps." @default.
- W1799231693 created "2016-06-24" @default.
- W1799231693 creator A5009816443 @default.
- W1799231693 creator A5086380170 @default.
- W1799231693 date "2012-06-19" @default.
- W1799231693 modified "2023-09-25" @default.
- W1799231693 title "The World of “Omics”: Genomics, Transcriptomics, Proteomics, and Metabolomics" @default.
- W1799231693 doi "https://doi.org/10.1017/cbo9780511811227.005" @default.
- W1799231693 hasPublicationYear "2012" @default.
- W1799231693 type Work @default.
- W1799231693 sameAs 1799231693 @default.
- W1799231693 citedByCount "0" @default.
- W1799231693 crossrefType "book-chapter" @default.
- W1799231693 hasAuthorship W1799231693A5009816443 @default.
- W1799231693 hasAuthorship W1799231693A5086380170 @default.
- W1799231693 hasBestOaLocation W17992316931 @default.
- W1799231693 hasConcept C101985253 @default.
- W1799231693 hasConcept C104317684 @default.
- W1799231693 hasConcept C113425843 @default.
- W1799231693 hasConcept C132917006 @default.
- W1799231693 hasConcept C137858568 @default.
- W1799231693 hasConcept C141231307 @default.
- W1799231693 hasConcept C15151743 @default.
- W1799231693 hasConcept C189206191 @default.
- W1799231693 hasConcept C192800701 @default.
- W1799231693 hasConcept C192953774 @default.
- W1799231693 hasConcept C205270622 @default.
- W1799231693 hasConcept C24432333 @default.
- W1799231693 hasConcept C51679486 @default.
- W1799231693 hasConcept C54355233 @default.
- W1799231693 hasConcept C70721500 @default.
- W1799231693 hasConcept C76818968 @default.
- W1799231693 hasConcept C86803240 @default.
- W1799231693 hasConceptScore W1799231693C101985253 @default.
- W1799231693 hasConceptScore W1799231693C104317684 @default.
- W1799231693 hasConceptScore W1799231693C113425843 @default.
- W1799231693 hasConceptScore W1799231693C132917006 @default.
- W1799231693 hasConceptScore W1799231693C137858568 @default.
- W1799231693 hasConceptScore W1799231693C141231307 @default.
- W1799231693 hasConceptScore W1799231693C15151743 @default.
- W1799231693 hasConceptScore W1799231693C189206191 @default.
- W1799231693 hasConceptScore W1799231693C192800701 @default.
- W1799231693 hasConceptScore W1799231693C192953774 @default.
- W1799231693 hasConceptScore W1799231693C205270622 @default.
- W1799231693 hasConceptScore W1799231693C24432333 @default.
- W1799231693 hasConceptScore W1799231693C51679486 @default.
- W1799231693 hasConceptScore W1799231693C54355233 @default.
- W1799231693 hasConceptScore W1799231693C70721500 @default.
- W1799231693 hasConceptScore W1799231693C76818968 @default.
- W1799231693 hasConceptScore W1799231693C86803240 @default.
- W1799231693 hasLocation W17992316931 @default.
- W1799231693 hasOpenAccess W1799231693 @default.
- W1799231693 hasPrimaryLocation W17992316931 @default.
- W1799231693 hasRelatedWork W120227485 @default.
- W1799231693 hasRelatedWork W1474375804 @default.
- W1799231693 hasRelatedWork W1579467649 @default.
- W1799231693 hasRelatedWork W1596938902 @default.
- W1799231693 hasRelatedWork W1603286518 @default.
- W1799231693 hasRelatedWork W1920924307 @default.
- W1799231693 hasRelatedWork W2091376961 @default.
- W1799231693 hasRelatedWork W2096095837 @default.
- W1799231693 hasRelatedWork W2113310577 @default.
- W1799231693 hasRelatedWork W2158506219 @default.
- W1799231693 hasRelatedWork W2214777088 @default.
- W1799231693 hasRelatedWork W2228443079 @default.
- W1799231693 hasRelatedWork W2315606519 @default.
- W1799231693 hasRelatedWork W2410064213 @default.
- W1799231693 hasRelatedWork W284499227 @default.
- W1799231693 hasRelatedWork W3033028902 @default.
- W1799231693 hasRelatedWork W3047163950 @default.
- W1799231693 hasRelatedWork W56844758 @default.
- W1799231693 hasRelatedWork W949590865 @default.
- W1799231693 hasRelatedWork W2579981877 @default.
- W1799231693 isParatext "false" @default.
- W1799231693 isRetracted "false" @default.
- W1799231693 magId "1799231693" @default.
- W1799231693 workType "book-chapter" @default.