Matches in SemOpenAlex for { <https://semopenalex.org/work/W1799542790> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W1799542790 endingPage "402" @default.
- W1799542790 startingPage "397" @default.
- W1799542790 abstract "A novel radiochemical assay for long-chain fatty acid:CoASH ligase activity (AMP) (EC 6.2.1.3) has been developed based on the conversion of [3H]CoASH to long-chain fatty acyl CoA. Fatty acyl [3H]CoA was quantitatively retained on Millipore filters upon filtration of the acidified reaction mixture under conditions where the [3H]CoASH was not retained. The assay was developed using microsomes derived from isolated fat cells as the source of fatty acid:CoASH ligase activity. The assay performed at 25 degrees C for 10 min was linear with added microsomal protein up to 7 mug. The assay was linear with time up to 24 min when 1 mug of protein was employed. Fatty acid:CoASH ligase activity was strongly dependent on ATP and magnesium, was stimulated by Triton WR-1339, and was two- to fivefold dependent on added fatty acid. The filter assay is easier than existing assays based on incorporation of labeled fatty acid and is equally sensitive." @default.
- W1799542790 created "2016-06-24" @default.
- W1799542790 creator A5031110077 @default.
- W1799542790 creator A5066952986 @default.
- W1799542790 date "1975-09-01" @default.
- W1799542790 modified "2023-10-17" @default.
- W1799542790 title "Millipore filter assay for long-chain fatty acid:CoASH ligase activity using 3H-labeled coenzyme A." @default.
- W1799542790 cites W1501304658 @default.
- W1799542790 cites W1517529383 @default.
- W1799542790 cites W1593480024 @default.
- W1799542790 cites W1596582715 @default.
- W1799542790 cites W1933696555 @default.
- W1799542790 cites W1967676656 @default.
- W1799542790 cites W1977450387 @default.
- W1799542790 cites W1986742090 @default.
- W1799542790 cites W2026912546 @default.
- W1799542790 cites W2031943127 @default.
- W1799542790 cites W2064748020 @default.
- W1799542790 cites W2065079376 @default.
- W1799542790 cites W2078261519 @default.
- W1799542790 cites W2082517041 @default.
- W1799542790 cites W2082558945 @default.
- W1799542790 cites W2083928713 @default.
- W1799542790 cites W2143873575 @default.
- W1799542790 cites W2213082044 @default.
- W1799542790 cites W2220565486 @default.
- W1799542790 cites W2415400738 @default.
- W1799542790 cites W48316491 @default.
- W1799542790 cites W2017687155 @default.
- W1799542790 doi "https://doi.org/10.1016/s0022-2275(20)34533-8" @default.
- W1799542790 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1176836" @default.
- W1799542790 hasPublicationYear "1975" @default.
- W1799542790 type Work @default.
- W1799542790 sameAs 1799542790 @default.
- W1799542790 citedByCount "28" @default.
- W1799542790 countsByYear W17995427902017 @default.
- W1799542790 crossrefType "journal-article" @default.
- W1799542790 hasAuthorship W1799542790A5031110077 @default.
- W1799542790 hasAuthorship W1799542790A5066952986 @default.
- W1799542790 hasBestOaLocation W17995427901 @default.
- W1799542790 hasConcept C134651460 @default.
- W1799542790 hasConcept C175114707 @default.
- W1799542790 hasConcept C181199279 @default.
- W1799542790 hasConcept C185592680 @default.
- W1799542790 hasConcept C197957613 @default.
- W1799542790 hasConcept C2779123261 @default.
- W1799542790 hasConcept C2779685201 @default.
- W1799542790 hasConcept C543025807 @default.
- W1799542790 hasConcept C55493867 @default.
- W1799542790 hasConceptScore W1799542790C134651460 @default.
- W1799542790 hasConceptScore W1799542790C175114707 @default.
- W1799542790 hasConceptScore W1799542790C181199279 @default.
- W1799542790 hasConceptScore W1799542790C185592680 @default.
- W1799542790 hasConceptScore W1799542790C197957613 @default.
- W1799542790 hasConceptScore W1799542790C2779123261 @default.
- W1799542790 hasConceptScore W1799542790C2779685201 @default.
- W1799542790 hasConceptScore W1799542790C543025807 @default.
- W1799542790 hasConceptScore W1799542790C55493867 @default.
- W1799542790 hasIssue "5" @default.
- W1799542790 hasLocation W17995427901 @default.
- W1799542790 hasOpenAccess W1799542790 @default.
- W1799542790 hasPrimaryLocation W17995427901 @default.
- W1799542790 hasRelatedWork W1201389745 @default.
- W1799542790 hasRelatedWork W1638675245 @default.
- W1799542790 hasRelatedWork W169735115 @default.
- W1799542790 hasRelatedWork W1712466180 @default.
- W1799542790 hasRelatedWork W1970500939 @default.
- W1799542790 hasRelatedWork W1976862950 @default.
- W1799542790 hasRelatedWork W2002173318 @default.
- W1799542790 hasRelatedWork W2019579035 @default.
- W1799542790 hasRelatedWork W2093620670 @default.
- W1799542790 hasRelatedWork W2203757003 @default.
- W1799542790 hasVolume "16" @default.
- W1799542790 isParatext "false" @default.
- W1799542790 isRetracted "false" @default.
- W1799542790 magId "1799542790" @default.
- W1799542790 workType "article" @default.