FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1799651673> ?p ?o ?g. }

• W1799651673 endingPage "333" @default.
• W1799651673 startingPage "323" @default.
• W1799651673 abstract "Abstract The luminescent Eu III ion has been used to probe the metal‐binding sites of bovine α‐lactalbumin (BLA) in D 2 O. Upon addition of apo‐BLA to an Eu III ‐containing solution, the intrinsic luminescence of the protein is quenched, and the Eu III luminescence is enhanced. Luminescent titrations point to there being at least two different metal‐binding sites in the apo‐protein. Curve analysis of the high resolution 5 D 0 ← 7 F 0 excitation spectra reveals the existence of three different environments for the bonded Eu III ions. Two environments, labelled I a and I b , give 5 D 0 ← 7 F 0 bands very close in energy; they contain four negatively charged groups and are assigned to one site we identify as the calcium‐binding site. Site I is protected from solvent influences and is somewhat rigid, since it displays selectivity towards lanthanide ions. The origin of the two similar environments I a and I b could not be determined unambiguously. The third environment is ascribed to a nonspecific metal‐binding site in which the Eu III ion is more exposed to the solvent (site II). It is sequentially populated after saturation of site I, and its population is pH‐dependent. The affinity constant of Eu III for this site was estimated from the excitation spectra: log K 2 app  3.5(1). Assignment of the metal binding sites has been facilitated by comparison with model compounds, [Eu(dota)] − (dota  1,4,7,10‐tetraazacyclododecane N,N′,N″, N‴ ‐tetraacetate), [Eu(dtpa)] 2− (dtpa  diethylenetriamine tetraacetate), and [Eu(bsa)] (bsa  bovine serum albumin). The usefulness and limits of the use of curve‐analysis procedures to unravel the various components of 5 D 0 ← 7 F 0 excitation spectra in biological materials are also discussed." @default.
• W1799651673 created "2016-06-24" @default.
• W1799651673 creator A5021528466 @default.
• W1799651673 creator A5029986895 @default.
• W1799651673 date "1994-02-09" @default.
• W1799651673 modified "2023-09-29" @default.
• W1799651673 title "Bovine α-Lactalbumin: Identification of two metal-ion-binding sites using the europium (III) luminescent probe" @default.
• W1799651673 cites W1483589957 @default.
• W1799651673 cites W1545856361 @default.
• W1799651673 cites W1553328469 @default.
• W1799651673 cites W1574212768 @default.
• W1799651673 cites W1583421526 @default.
• W1799651673 cites W1606101287 @default.
• W1799651673 cites W1691583558 @default.
• W1799651673 cites W1965509947 @default.
• W1799651673 cites W1970712942 @default.
• W1799651673 cites W1984610316 @default.
• W1799651673 cites W1990983671 @default.
• W1799651673 cites W2007048003 @default.
• W1799651673 cites W2034231595 @default.
• W1799651673 cites W2039558304 @default.
• W1799651673 cites W2058236594 @default.
• W1799651673 cites W2076166333 @default.
• W1799651673 cites W2076907050 @default.
• W1799651673 cites W2078074216 @default.
• W1799651673 cites W2085417139 @default.
• W1799651673 cites W2086186602 @default.
• W1799651673 cites W2089778751 @default.
• W1799651673 cites W2140114637 @default.
• W1799651673 cites W2152862781 @default.
• W1799651673 cites W2400904451 @default.
• W1799651673 doi "https://doi.org/10.1002/hlca.19940770132" @default.
• W1799651673 hasPublicationYear "1994" @default.
• W1799651673 type Work @default.
• W1799651673 sameAs 1799651673 @default.
• W1799651673 citedByCount "16" @default.
• W1799651673 crossrefType "journal-article" @default.
• W1799651673 hasAuthorship W1799651673A5021528466 @default.
• W1799651673 hasAuthorship W1799651673A5029986895 @default.
• W1799651673 hasConcept C107824862 @default.
• W1799651673 hasConcept C113196181 @default.
• W1799651673 hasConcept C121332964 @default.
• W1799651673 hasConcept C144024400 @default.
• W1799651673 hasConcept C145148216 @default.
• W1799651673 hasConcept C148869448 @default.
• W1799651673 hasConcept C149923435 @default.
• W1799651673 hasConcept C178790620 @default.
• W1799651673 hasConcept C179104552 @default.
• W1799651673 hasConcept C184866935 @default.
• W1799651673 hasConcept C185592680 @default.
• W1799651673 hasConcept C199164860 @default.
• W1799651673 hasConcept C201770740 @default.
• W1799651673 hasConcept C2777459166 @default.
• W1799651673 hasConcept C2908647359 @default.
• W1799651673 hasConcept C32786932 @default.
• W1799651673 hasConcept C49040817 @default.
• W1799651673 hasConcept C502696234 @default.
• W1799651673 hasConcept C544153396 @default.
• W1799651673 hasConcept C55493867 @default.
• W1799651673 hasConcept C62520636 @default.
• W1799651673 hasConcept C71240020 @default.
• W1799651673 hasConcept C8010536 @default.
• W1799651673 hasConcept C91881484 @default.
• W1799651673 hasConceptScore W1799651673C107824862 @default.
• W1799651673 hasConceptScore W1799651673C113196181 @default.
• W1799651673 hasConceptScore W1799651673C121332964 @default.
• W1799651673 hasConceptScore W1799651673C144024400 @default.
• W1799651673 hasConceptScore W1799651673C145148216 @default.
• W1799651673 hasConceptScore W1799651673C148869448 @default.
• W1799651673 hasConceptScore W1799651673C149923435 @default.
• W1799651673 hasConceptScore W1799651673C178790620 @default.
• W1799651673 hasConceptScore W1799651673C179104552 @default.
• W1799651673 hasConceptScore W1799651673C184866935 @default.
• W1799651673 hasConceptScore W1799651673C185592680 @default.
• W1799651673 hasConceptScore W1799651673C199164860 @default.
• W1799651673 hasConceptScore W1799651673C201770740 @default.
• W1799651673 hasConceptScore W1799651673C2777459166 @default.
• W1799651673 hasConceptScore W1799651673C2908647359 @default.
• W1799651673 hasConceptScore W1799651673C32786932 @default.
• W1799651673 hasConceptScore W1799651673C49040817 @default.
• W1799651673 hasConceptScore W1799651673C502696234 @default.
• W1799651673 hasConceptScore W1799651673C544153396 @default.
• W1799651673 hasConceptScore W1799651673C55493867 @default.
• W1799651673 hasConceptScore W1799651673C62520636 @default.
• W1799651673 hasConceptScore W1799651673C71240020 @default.
• W1799651673 hasConceptScore W1799651673C8010536 @default.
• W1799651673 hasConceptScore W1799651673C91881484 @default.
• W1799651673 hasIssue "1" @default.
• W1799651673 hasLocation W17996516731 @default.
• W1799651673 hasOpenAccess W1799651673 @default.
• W1799651673 hasPrimaryLocation W17996516731 @default.
• W1799651673 hasRelatedWork W1982803414 @default.
• W1799651673 hasRelatedWork W1991971557 @default.
• W1799651673 hasRelatedWork W2002662809 @default.
• W1799651673 hasRelatedWork W2049465508 @default.
• W1799651673 hasRelatedWork W2060141671 @default.
• W1799651673 hasRelatedWork W2065614488 @default.
• W1799651673 hasRelatedWork W2744199062 @default.

• next