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- W1799782526 abstract "This chapter discusses that both viral and nonviral inducers can be used in the preparation of rabbit serum interferon. Several viruses, including influenza A, newcastle disease, eastern equine encephalitis, and sindbis, are shown to be effective inducers in vivo. The experience with viral inducers has been primarily with Newcastle disease virus (NDV), and the experience with nonviral inducers has been limited to endotoxin and the double-stranded polyribonucleotide poly(I).poly(C). Interferon induced by NDV produced by RK13 cells is heterogeneous by isoelectric focusing with six peaks from pH 5.07 to 6.68. When rabbit interferon is treated with neuraminidase, such asialointerferon, it gets homogeneous in charge and of undiminished biological activity. Enzymic incorporation of N-acetylneuramic acid restored heterogeneity. It is concluded that rabbit interferon is a glycoprotein with the terminal oligosaccharide sequence sialic acid-galactose. The decreased responsiveness in interferon production when cell cultures or animals are reexposed to an interferon inducer is termed as “hyporeactivity.”" @default.
- W1799782526 created "2016-06-24" @default.
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- W1799782526 date "1981-01-01" @default.
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- W1799782526 title "[16] Preparation and characterization of rabbit interferon" @default.
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- W1799782526 doi "https://doi.org/10.1016/0076-6879(81)78106-0" @default.
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