Matches in SemOpenAlex for { <https://semopenalex.org/work/W1800771389> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1800771389 endingPage "640" @default.
- W1800771389 startingPage "636" @default.
- W1800771389 abstract "Objective:To study the effect of Bcl-2 short hairpin RNA (shRNA) in enhancing methotrexate (MTX)-induced apoptosis of Raji cells. Methods:Expression plasmid containing Bcl-2 shRNA was transfected into Raji cells by lipofectmine 2000 and then the transfected cells were treated with MTX. The expression levels of Bcl-2 mRNA and protein were evaluated by RT-PCR and immunofluorescence method at 48 h of transfection. MTT assay was used to analyze cell proliferation at 24, 48 and 72 h. Apoptosis was detected by Giemsa staining and flow cytomertric cell cycle analysis. Results:After transfection with Bcl-2 shRNA, the expression levels of Bcl-2 mRNA and protein in Raji cells were significantly decreased (P0.05). Bcl-2 shRNA transfection plus MTX treatment induced marked apoptosis, decreased in cell proliferation activity, and increased in apoptotic rate. The difference was significant compared with MTX group, negative shRNA plus MTX group, Bcl-2 shRNA group, and empty plasmid plus MTX group (P0.05). Conclusion:Bcl-2 shRNA could enhance MTX-induced apoptosis and inhibition of cell proliferation in Raji cells." @default.
- W1800771389 created "2016-06-24" @default.
- W1800771389 creator A5018529469 @default.
- W1800771389 creator A5032447166 @default.
- W1800771389 creator A5038495757 @default.
- W1800771389 creator A5086783805 @default.
- W1800771389 date "2009-01-01" @default.
- W1800771389 modified "2023-09-24" @default.
- W1800771389 title "The study on effects of Bcl-2 shRNA in enhancing methotrexate-induced apoptosis and inhibiting cell proliferation in Raji cells." @default.
- W1800771389 hasPublicationYear "2009" @default.
- W1800771389 type Work @default.
- W1800771389 sameAs 1800771389 @default.
- W1800771389 citedByCount "0" @default.
- W1800771389 crossrefType "journal-article" @default.
- W1800771389 hasAuthorship W1800771389A5018529469 @default.
- W1800771389 hasAuthorship W1800771389A5032447166 @default.
- W1800771389 hasAuthorship W1800771389A5038495757 @default.
- W1800771389 hasAuthorship W1800771389A5086783805 @default.
- W1800771389 hasConcept C1491633281 @default.
- W1800771389 hasConcept C153911025 @default.
- W1800771389 hasConcept C16232356 @default.
- W1800771389 hasConcept C173396325 @default.
- W1800771389 hasConcept C185592680 @default.
- W1800771389 hasConcept C190232843 @default.
- W1800771389 hasConcept C190283241 @default.
- W1800771389 hasConcept C2780783641 @default.
- W1800771389 hasConcept C29537977 @default.
- W1800771389 hasConcept C54009773 @default.
- W1800771389 hasConcept C54355233 @default.
- W1800771389 hasConcept C553184892 @default.
- W1800771389 hasConcept C55493867 @default.
- W1800771389 hasConcept C62112901 @default.
- W1800771389 hasConcept C81885089 @default.
- W1800771389 hasConcept C86803240 @default.
- W1800771389 hasConceptScore W1800771389C1491633281 @default.
- W1800771389 hasConceptScore W1800771389C153911025 @default.
- W1800771389 hasConceptScore W1800771389C16232356 @default.
- W1800771389 hasConceptScore W1800771389C173396325 @default.
- W1800771389 hasConceptScore W1800771389C185592680 @default.
- W1800771389 hasConceptScore W1800771389C190232843 @default.
- W1800771389 hasConceptScore W1800771389C190283241 @default.
- W1800771389 hasConceptScore W1800771389C2780783641 @default.
- W1800771389 hasConceptScore W1800771389C29537977 @default.
- W1800771389 hasConceptScore W1800771389C54009773 @default.
- W1800771389 hasConceptScore W1800771389C54355233 @default.
- W1800771389 hasConceptScore W1800771389C553184892 @default.
- W1800771389 hasConceptScore W1800771389C55493867 @default.
- W1800771389 hasConceptScore W1800771389C62112901 @default.
- W1800771389 hasConceptScore W1800771389C81885089 @default.
- W1800771389 hasConceptScore W1800771389C86803240 @default.
- W1800771389 hasIssue "7" @default.
- W1800771389 hasLocation W18007713891 @default.
- W1800771389 hasOpenAccess W1800771389 @default.
- W1800771389 hasPrimaryLocation W18007713891 @default.
- W1800771389 hasRelatedWork W1988197509 @default.
- W1800771389 hasRelatedWork W2013212941 @default.
- W1800771389 hasRelatedWork W2052971130 @default.
- W1800771389 hasRelatedWork W2070849774 @default.
- W1800771389 hasRelatedWork W2073449334 @default.
- W1800771389 hasRelatedWork W2098423522 @default.
- W1800771389 hasRelatedWork W2376130496 @default.
- W1800771389 hasRelatedWork W2377999420 @default.
- W1800771389 hasRelatedWork W2405815479 @default.
- W1800771389 hasRelatedWork W2412750807 @default.
- W1800771389 hasRelatedWork W2414720169 @default.
- W1800771389 hasRelatedWork W2417790292 @default.
- W1800771389 hasRelatedWork W2433636251 @default.
- W1800771389 hasRelatedWork W2438394703 @default.
- W1800771389 hasRelatedWork W2440528242 @default.
- W1800771389 hasRelatedWork W2443234167 @default.
- W1800771389 hasRelatedWork W2564056943 @default.
- W1800771389 hasRelatedWork W2589520965 @default.
- W1800771389 hasRelatedWork W2594625063 @default.
- W1800771389 hasRelatedWork W3140416643 @default.
- W1800771389 hasVolume "29" @default.
- W1800771389 isParatext "false" @default.
- W1800771389 isRetracted "false" @default.
- W1800771389 magId "1800771389" @default.
- W1800771389 workType "article" @default.