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• W1801362421 endingPage "98" @default.
• W1801362421 startingPage "1980" @default.
• W1801362421 abstract "Since Watson and Crick's historical papers on the structure and function of DNA based on Rosalind Franklin's and Maurice Wilkin's X-ray diffraction patterns tremendous scientific curiosity has been aroused by the unique and dynamic structure of the molecule of life. A-DNA and B-DNA represent different conformations of the DNA molecule, which is stabilised by hydrogen interactions between base pairs, stacking interactions between neighboring bases and long-range intra- and inter-backbone forces. This review highlights the contribution Fourier transform infrared (FTIR) spectroscopy has made to the understanding of DNA conformation in relation to hydration and its potential role in clinical diagnostics. The review will first begin by elucidating the main forms of DNA conformation found in nature and the general structures of the A, B and Z forms. This is followed by a detailed critique on infrared spectroscopy applied to DNA conformation highlighting pivotal studies on isolated DNA, polynucleotides, nucleoprotein and nucleohistone complexes. A discussion on the potential of diagnosing cancer using FTIR spectroscopy based on the detection of DNA bands in cells and tissues will ensue, highlighting the recent studies investigating the conformation of DNA in hydrated and dehydrated cells. The method of hydration as a way to facilitate DNA conformational band assignment will be discussed and the conformational change to the A-form upon dehydration will be used to explain the reason for the apparent lack of FTIR DNA signals observed in fixed or air-dried cells and tissues. The advantages of investigating B-DNA in the hydrated state, as opposed to A-DNA in the dehydrated state, are exemplified in a series of studies that show: (1) improved quantification of DNA in cells; (2) improved discrimination and reproducibility of FTIR spectra recorded of cells progressing through the cell cycle; (3) insights into the biological significance of A-DNA as evidenced by an interesting study on bacteria, which can survive desiccation and at the same time undergo the B-A-B transition. Finally, the importance of preserving the B-DNA conformation for the diagnosis of cancer is put forward as way to improve the sensitivity of this powerful technique." @default.
• W1801362421 created "2016-06-24" @default.
• W1801362421 creator A5044253971 @default.
• W1801362421 date "2016-04-07" @default.
• W1801362421 modified "2023-09-29" @default.
• W1801362421 title "The importance of hydration and DNA conformation in interpreting infrared spectra of cells and tissues." @default.
• W1801362421 cites W1030900860 @default.
• W1801362421 cites W1149146151 @default.
• W1801362421 cites W131840713 @default.
• W1801362421 cites W1501369544 @default.
• W1801362421 cites W1511556470 @default.
• W1801362421 cites W1543277803 @default.
• W1801362421 cites W1544851750 @default.
• W1801362421 cites W1563722950 @default.
• W1801362421 cites W1587493794 @default.
• W1801362421 cites W1605738254 @default.
• W1801362421 cites W1791966007 @default.
• W1801362421 cites W1841037392 @default.
• W1801362421 cites W1901320169 @default.
• W1801362421 cites W1914825094 @default.
• W1801362421 cites W1964451198 @default.
• W1801362421 cites W1964642968 @default.
• W1801362421 cites W1965879885 @default.
• W1801362421 cites W1966296832 @default.
• W1801362421 cites W1967581268 @default.
• W1801362421 cites W1968187929 @default.
• W1801362421 cites W1968647654 @default.
• W1801362421 cites W1969528040 @default.
• W1801362421 cites W1971481046 @default.
• W1801362421 cites W1972698196 @default.
• W1801362421 cites W1973591923 @default.
• W1801362421 cites W1974488437 @default.
• W1801362421 cites W1975700737 @default.
• W1801362421 cites W1975855471 @default.
• W1801362421 cites W1975902603 @default.
• W1801362421 cites W1976288860 @default.
• W1801362421 cites W1977615915 @default.
• W1801362421 cites W1979270110 @default.
• W1801362421 cites W1980972026 @default.
• W1801362421 cites W1982083021 @default.
• W1801362421 cites W1984858613 @default.
• W1801362421 cites W1986399871 @default.
• W1801362421 cites W1986628337 @default.
• W1801362421 cites W1990759616 @default.
• W1801362421 cites W1990894350 @default.
• W1801362421 cites W1993069380 @default.
• W1801362421 cites W1994323940 @default.
• W1801362421 cites W1995008811 @default.
• W1801362421 cites W1996708937 @default.
• W1801362421 cites W1996743857 @default.
• W1801362421 cites W1999300829 @default.
• W1801362421 cites W1999550833 @default.
• W1801362421 cites W2002716793 @default.
• W1801362421 cites W2003857638 @default.
• W1801362421 cites W2004982513 @default.
• W1801362421 cites W2006364473 @default.
• W1801362421 cites W2007831485 @default.
• W1801362421 cites W2009581957 @default.
• W1801362421 cites W2011587432 @default.
• W1801362421 cites W2012171819 @default.
• W1801362421 cites W2012636089 @default.
• W1801362421 cites W2017959781 @default.
• W1801362421 cites W2019251428 @default.
• W1801362421 cites W2023752722 @default.
• W1801362421 cites W2025187077 @default.
• W1801362421 cites W2029037656 @default.
• W1801362421 cites W2029141607 @default.
• W1801362421 cites W2030474621 @default.
• W1801362421 cites W2032289589 @default.
• W1801362421 cites W2033248991 @default.
• W1801362421 cites W2033342471 @default.
• W1801362421 cites W2033700815 @default.
• W1801362421 cites W2034825360 @default.
• W1801362421 cites W2037306230 @default.
• W1801362421 cites W2038921023 @default.
• W1801362421 cites W2040135775 @default.
• W1801362421 cites W2043076197 @default.
• W1801362421 cites W2044348179 @default.
• W1801362421 cites W2046688097 @default.
• W1801362421 cites W2050393537 @default.
• W1801362421 cites W2050402446 @default.
• W1801362421 cites W2050816779 @default.
• W1801362421 cites W2055003378 @default.
• W1801362421 cites W2055277159 @default.
• W1801362421 cites W2057588337 @default.
• W1801362421 cites W2059528376 @default.
• W1801362421 cites W2062982616 @default.
• W1801362421 cites W2064194732 @default.
• W1801362421 cites W2065430807 @default.
• W1801362421 cites W2066485967 @default.
• W1801362421 cites W2067103174 @default.
• W1801362421 cites W2069243797 @default.
• W1801362421 cites W2071686507 @default.
• W1801362421 cites W2071893949 @default.
• W1801362421 cites W2072249969 @default.
• W1801362421 cites W2072888261 @default.
• W1801362421 cites W2074392294 @default.
• W1801362421 cites W2074455132 @default.

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