Matches in SemOpenAlex for { <https://semopenalex.org/work/W1801996848> ?p ?o ?g. }
Showing items 1 to 42 of
42
with 100 items per page.
- W1801996848 abstract "Lignocellulose-acting enzymes are often appended to one or more carbohydrate binding modules (CBMs) which promote substrate targeting due to their variable substrate specificity and affinity. They thus play a central role in the enzymatic deconstruction of plant cell wall biomass, but their fine characterization is generally performed with pure isolated polymers which do not reflect the complex plant cell walls. In order to gain more information on the behavior and mobility of CBMs in complex 3D substrates, we have devised and used bioinspired model assemblies that contain some of the polymers and covalent interactions found in plant cell walls. These assemblies contain feruloylated arabinoxylans (FAXs) and cellulose nano-crystals (CNCs) at various concentrations in solution (free polymers) or in gels (cross-linked polymers). The three CBMs studied herein belong to family 4 and have been engineered so that they have different affinities and specificities toward FAX and CNC: CBM4-2 binds FAX and CNC, CBM X-2 binds only FAX and CBM G-4 binds neither FAX nor CNC. These CBMs have been produced as monomers and as dimers and then been grafted to a fluorophore so that their mobility in the bioinspired assemblies can be followed by measuring the fluorescence recovery after photobleaching. By varying three parameters related to the assemblies (CNC concentration, solution or gel state) and to the CBMs (oligomerisation state), we have been able to calculate an apparent affinity and to model the mobility of the CBMs. Overall, our results reveal that apparent affinity is drastically modified in a 3D polymer network in comparison to their behavior in solution, and parameters varied influence differently each CBM. This demonstrates that bioinspired assemblies may provide important information relevant for studies of carbohydrate active proteins such as the selection of appropriate CBM constructs to be further exploited in applications targeting complex carbohydrate substrates." @default.
- W1801996848 created "2016-06-24" @default.
- W1801996848 creator A5065666731 @default.
- W1801996848 creator A5072019967 @default.
- W1801996848 creator A5091376342 @default.
- W1801996848 date "2014-03-16" @default.
- W1801996848 modified "2023-10-14" @default.
- W1801996848 title "New insights into mobility and affinity of engineered family 4 CBMs in bioinspired lignocellulosic assemblies" @default.
- W1801996848 hasPublicationYear "2014" @default.
- W1801996848 type Work @default.
- W1801996848 sameAs 1801996848 @default.
- W1801996848 citedByCount "0" @default.
- W1801996848 crossrefType "proceedings-article" @default.
- W1801996848 hasAuthorship W1801996848A5065666731 @default.
- W1801996848 hasAuthorship W1801996848A5072019967 @default.
- W1801996848 hasAuthorship W1801996848A5091376342 @default.
- W1801996848 hasConcept C127413603 @default.
- W1801996848 hasConcept C171250308 @default.
- W1801996848 hasConcept C183696295 @default.
- W1801996848 hasConcept C192562407 @default.
- W1801996848 hasConceptScore W1801996848C127413603 @default.
- W1801996848 hasConceptScore W1801996848C171250308 @default.
- W1801996848 hasConceptScore W1801996848C183696295 @default.
- W1801996848 hasConceptScore W1801996848C192562407 @default.
- W1801996848 hasLocation W18019968481 @default.
- W1801996848 hasLocation W18019968482 @default.
- W1801996848 hasOpenAccess W1801996848 @default.
- W1801996848 hasPrimaryLocation W18019968481 @default.
- W1801996848 hasRelatedWork W2018879842 @default.
- W1801996848 hasRelatedWork W2737498735 @default.
- W1801996848 hasRelatedWork W2744391499 @default.
- W1801996848 hasRelatedWork W2898370298 @default.
- W1801996848 hasRelatedWork W2899084033 @default.
- W1801996848 hasRelatedWork W3120461830 @default.
- W1801996848 hasRelatedWork W3126600910 @default.
- W1801996848 hasRelatedWork W4292492973 @default.
- W1801996848 hasRelatedWork W4293236590 @default.
- W1801996848 hasRelatedWork W4320909741 @default.
- W1801996848 isParatext "false" @default.
- W1801996848 isRetracted "false" @default.
- W1801996848 magId "1801996848" @default.
- W1801996848 workType "article" @default.