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• W1802472992 endingPage "460" @default.
• W1802472992 startingPage "454" @default.
• W1802472992 abstract "The purpose of this study was to investigate the effect of 1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane (DDT), 1,1-bis-(chlorophenyl)-2,2-dichloroethene (DDE), and 1,1-dichloro-2,2-bis(chlorophenyl)ethane (DDD) isomers on COX-2 expression in a human trophoblast-derived cell line. Cultured HTR-8/SVneo trophoblast cells were exposed to DDT isomers and its metabolites for 24 h, and COX-2 mRNA and protein expression were assessed by RT-PCR, Western blotting, and ELISA. Prostaglandin E₂ production was also measured by ELISA. Both COX-2 mRNA and protein were detected under control (unexposed) conditions in the HTR-8/SVneo cell line. COX-2 protein expression and prostaglandin E₂ production but not COX-2 mRNA levels increased only after DDE and DDD isomers exposure. It is concluded that DDE and DDD exposure induce the expression of COX-2 protein, leading to increased prostaglandin E2 production. Interestingly, the regulation of COX-2 by these organochlorines pesticides appears to be at the translational level." @default.
• W1802472992 created "2016-06-24" @default.
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• W1802472992 date "2012-11-01" @default.
• W1802472992 modified "2023-10-17" @default.
• W1802472992 title "Differential Effect of DDT, DDE, and DDD on COX-2 Expression in the Human Trophoblast Derived HTR-8/SVneo Cells" @default.
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• W1802472992 doi "https://doi.org/10.1002/jbt.21444" @default.
• W1802472992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23132776" @default.
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