FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1802643526> ?p ?o ?g. }

• W1802643526 endingPage "766" @default.
• W1802643526 startingPage "751" @default.
• W1802643526 abstract "ABSTRACT Embryonic chick muscle cells were used to investigate the effect of removing cell-surface sialic acids on cell aggregation in vitro. Single cell suspensions were prepared by dissociating skeletal muscle tissue of 9-day-old chick embryos with either crystalline or crude trypsin. Cell aggregation was quantitatively estimated by turbidimetric and gyratory shaker methods. Cells dissociated with crude trypsin and suspended in Hanks’s balanced salts solution (BSS) containing 25 u./ml neuraminidase (NANase) only aggregated for 2 h when rotated in an absorptiometer. The inhibitory effect of the enzyme was more pronounced with increasing concentration up to 25 u./ml. Cells dissociated with crystalline trypsin and treated with 100 u./ml NANase immediately exhibited a reduced aggregative competence when gyrated in Eagle’s minimum essential medium (MEM) containing 25 u./ml NANase, compared with the controls which were not exposed to NANase. The aggregation rate of muscle cells pretreated with 100 u./ml NANase and suspended in Eagle’s MEM was similar to that of the untreated controls. Cell counts showed that under all three experimental conditions cells were not added to aggregates after the 12-h stage. Aggregates formed in Eagle’s MEM (the controls) joined together to form larger aggregates after 12 h, but those rotating in the presence of NANase did not display this property. Lissamine green viability tests showed that cells remained alive throughout the 24-h period in the presence of NANase. Determinations of oxygen uptake, protein synthesis and mitotic index confirmed that general cellular viability was not affected by NANase. Fluorescent-labelled NANase was not taken up by the cells. Treatment of crystalline trypsin-dissociated muscle cells with 100 u./ml NANase for 30 min at 37 °C significantly reduced their negative electrophoretic mobility. This diminution closely corresponded to the removal of cell-surface sialic acids, as measured by colorimetric tests. Interpretation of the results in the light of current theories of cell adhesion failed to give support to the concept of adhesion by physical forces. The mechanism by which cellular deformability could influence cellular adhesiveness is modified in the knowledge of the present results." @default.
• W1802643526 created "2016-06-24" @default.
• W1802643526 creator A5049034599 @default.
• W1802643526 date "1970-05-01" @default.
• W1802643526 modified "2023-10-18" @default.
• W1802643526 title "The Effect of Neuraminidase (3:2:1:18) on the Aggregation of Cells Dissociated from Embryonic Chick Muscle Tissue" @default.
• W1802643526 cites W1580023435 @default.
• W1802643526 cites W1598750898 @default.
• W1802643526 cites W1958395201 @default.
• W1802643526 cites W1969665472 @default.
• W1802643526 cites W1977145303 @default.
• W1802643526 cites W1978313011 @default.
• W1802643526 cites W1984078614 @default.
• W1802643526 cites W1993474219 @default.
• W1802643526 cites W1996421548 @default.
• W1802643526 cites W2011134898 @default.
• W1802643526 cites W2012214760 @default.
• W1802643526 cites W2012502160 @default.
• W1802643526 cites W2015660739 @default.
• W1802643526 cites W2029038499 @default.
• W1802643526 cites W2030326481 @default.
• W1802643526 cites W2041675819 @default.
• W1802643526 cites W2042201989 @default.
• W1802643526 cites W2042771331 @default.
• W1802643526 cites W2042995489 @default.
• W1802643526 cites W2050346102 @default.
• W1802643526 cites W2050486861 @default.
• W1802643526 cites W2052800806 @default.
• W1802643526 cites W2055729401 @default.
• W1802643526 cites W2056638162 @default.
• W1802643526 cites W2058321157 @default.
• W1802643526 cites W2061604932 @default.
• W1802643526 cites W2077019112 @default.
• W1802643526 cites W2084455283 @default.
• W1802643526 cites W2085136816 @default.
• W1802643526 cites W2108307012 @default.
• W1802643526 cites W2115752921 @default.
• W1802643526 cites W2128879732 @default.
• W1802643526 cites W2130081036 @default.
• W1802643526 cites W2137200531 @default.
• W1802643526 cites W2137205280 @default.
• W1802643526 cites W2146313801 @default.
• W1802643526 cites W2164976975 @default.
• W1802643526 cites W2270523344 @default.
• W1802643526 cites W2496383277 @default.
• W1802643526 cites W4323284205 @default.
• W1802643526 cites W4365786647 @default.
• W1802643526 cites W2032978012 @default.
• W1802643526 doi "https://doi.org/10.1242/jcs.6.3.751" @default.
• W1802643526 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5452093" @default.
• W1802643526 hasPublicationYear "1970" @default.
• W1802643526 type Work @default.
• W1802643526 sameAs 1802643526 @default.
• W1802643526 citedByCount "66" @default.
• W1802643526 countsByYear W18026435262012 @default.
• W1802643526 countsByYear W18026435262016 @default.
• W1802643526 countsByYear W18026435262020 @default.
• W1802643526 countsByYear W18026435262022 @default.
• W1802643526 crossrefType "journal-article" @default.
• W1802643526 hasAuthorship W1802643526A5049034599 @default.
• W1802643526 hasConcept C104317684 @default.
• W1802643526 hasConcept C105702510 @default.
• W1802643526 hasConcept C12554922 @default.
• W1802643526 hasConcept C134164806 @default.
• W1802643526 hasConcept C145103041 @default.
• W1802643526 hasConcept C1491633281 @default.
• W1802643526 hasConcept C153911025 @default.
• W1802643526 hasConcept C181199279 @default.
• W1802643526 hasConcept C2780115458 @default.
• W1802643526 hasConcept C2780340462 @default.
• W1802643526 hasConcept C53227056 @default.
• W1802643526 hasConcept C55493867 @default.
• W1802643526 hasConcept C86803240 @default.
• W1802643526 hasConceptScore W1802643526C104317684 @default.
• W1802643526 hasConceptScore W1802643526C105702510 @default.
• W1802643526 hasConceptScore W1802643526C12554922 @default.
• W1802643526 hasConceptScore W1802643526C134164806 @default.
• W1802643526 hasConceptScore W1802643526C145103041 @default.
• W1802643526 hasConceptScore W1802643526C1491633281 @default.
• W1802643526 hasConceptScore W1802643526C153911025 @default.
• W1802643526 hasConceptScore W1802643526C181199279 @default.
• W1802643526 hasConceptScore W1802643526C2780115458 @default.
• W1802643526 hasConceptScore W1802643526C2780340462 @default.
• W1802643526 hasConceptScore W1802643526C53227056 @default.
• W1802643526 hasConceptScore W1802643526C55493867 @default.
• W1802643526 hasConceptScore W1802643526C86803240 @default.
• W1802643526 hasIssue "3" @default.
• W1802643526 hasLocation W18026435261 @default.
• W1802643526 hasLocation W18026435262 @default.
• W1802643526 hasOpenAccess W1802643526 @default.
• W1802643526 hasPrimaryLocation W18026435261 @default.
• W1802643526 hasRelatedWork W1490269853 @default.
• W1802643526 hasRelatedWork W1784629112 @default.
• W1802643526 hasRelatedWork W1990237818 @default.
• W1802643526 hasRelatedWork W2006791845 @default.
• W1802643526 hasRelatedWork W2008111244 @default.
• W1802643526 hasRelatedWork W2029492475 @default.
• W1802643526 hasRelatedWork W2398447764 @default.

• next