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• W1803680760 endingPage "958" @default.
• W1803680760 startingPage "949" @default.
• W1803680760 abstract "A variety of nitroaromatic compounds, including 2,4,6-trinitrotoluene (TNT), were reduced by hydrogen in the presence of enzyme preparations from Veillonella alkalescens. Consistent with the proposed reduction pathway, R-NO2 H2 leads to R-NO H2 leads to R-NHOH H2 leads to R-NH2, 3 mol of H2 was utilized per mol of nitro group. The rates of reduction of 40 mono-, di-, and trinitroaromatic compounds by V. alkalescens extract were determined. The reactivity of the nitro groups depended on other substituents and on the position of the nitro groups relative to these substituents. In the case of the nitrotoluenes, the para-nitro group was the most readily reduced, the 4-nitro position of 2,4-dinitrotulene being reduced first. The pattern of reduction of TNT (disappearance of TNT and reduction products formed) depended on the type of preparation (cell-free extract, resting cells, or growing culture), on the species, and on the atmosphere (air or H2). The nitro-reductase activity of V. alkalescens extracts was associated with protein fractions, one having some ferredoxin-like properties and the other possessing hydrogenase activity. Efforts to eliminate hydrogenase from the reaction have thus far been unsuccessful. The question of whether ferredoxin acts as a nonspecific reductase for nitroaromatic compounds remains unresolved." @default.
• W1803680760 created "2016-06-24" @default.
• W1803680760 creator A5022211637 @default.
• W1803680760 creator A5042502946 @default.
• W1803680760 creator A5064460942 @default.
• W1803680760 date "1976-06-01" @default.
• W1803680760 modified "2023-10-12" @default.
• W1803680760 title "Microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds" @default.
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• W1803680760 doi "https://doi.org/10.1128/aem.31.6.949-958.1976" @default.
• W1803680760 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/169861" @default.
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