Matches in SemOpenAlex for { <https://semopenalex.org/work/W1805174755> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1805174755 startingPage "4484" @default.
- W1805174755 abstract "Introduction: IL27 is a cytokine secreted by antigen presenting cells and responsible mainly for the Th1 immune polarization and cytotoxic response. Because of its properties, IL27 may be a potential therapeutic tool for lung cancer immune therapy. Previously we constructed a plasmid encoding IL27 (pXMs-IL27).Aim: To evaluate a clinical potential of pXMs-IL27 as a therapeutic tool applied to human small- (H82) and non-small (A549) cell lung cancer lines.Materials and Methods: The expression of IL27 was performed by cell lines (A549 and H82) transfection with a pXMs-IL27 and empty control plasmid. Transfection efficacy was proved by RT-PCR and anti-IL27 indirect immunostaining. Additionally, the expression of CD80, CD83, CD86, CD120a, CD120b, CD178 molecules after transfection with plasmids was studied by direct immunofluorescence and analyzed by flow cytometry.Results: RT-PCR and flow cytometry confirmed the expression of IL27 in transfected cells (A549: 79%; H82: 56%, median of 5 experiments). With use of flow cytometry the baseline expression of IL27 was found in both cell lung cancer lines, particularly in A549 (40%). Tumor cells transfected with pXMs-IL27 plasmid showed in microscopic observations intense apoptosis, as compared with empty plasmid control.Conclusions: Cell lines were successfully transfected with pXMs-IL27 plasmid. Phenotypic changes and increased apoptosis rate in transfected cells suggest that IL27 will be useful in future immune therapy of lung cancer." @default.
- W1805174755 created "2016-06-24" @default.
- W1805174755 creator A5001105723 @default.
- W1805174755 creator A5013535597 @default.
- W1805174755 creator A5024514881 @default.
- W1805174755 creator A5035310424 @default.
- W1805174755 creator A5052478663 @default.
- W1805174755 creator A5068863201 @default.
- W1805174755 creator A5072789845 @default.
- W1805174755 creator A5085186140 @default.
- W1805174755 date "2013-09-01" @default.
- W1805174755 modified "2023-10-12" @default.
- W1805174755 title "Interleukin 27 (IL27) as a novel therapeutic tool in lung cancer immune therapy" @default.
- W1805174755 hasPublicationYear "2013" @default.
- W1805174755 type Work @default.
- W1805174755 sameAs 1805174755 @default.
- W1805174755 citedByCount "0" @default.
- W1805174755 crossrefType "journal-article" @default.
- W1805174755 hasAuthorship W1805174755A5001105723 @default.
- W1805174755 hasAuthorship W1805174755A5013535597 @default.
- W1805174755 hasAuthorship W1805174755A5024514881 @default.
- W1805174755 hasAuthorship W1805174755A5035310424 @default.
- W1805174755 hasAuthorship W1805174755A5052478663 @default.
- W1805174755 hasAuthorship W1805174755A5068863201 @default.
- W1805174755 hasAuthorship W1805174755A5072789845 @default.
- W1805174755 hasAuthorship W1805174755A5085186140 @default.
- W1805174755 hasConcept C104317684 @default.
- W1805174755 hasConcept C143998085 @default.
- W1805174755 hasConcept C153911025 @default.
- W1805174755 hasConcept C154317977 @default.
- W1805174755 hasConcept C190283241 @default.
- W1805174755 hasConcept C202751555 @default.
- W1805174755 hasConcept C203014093 @default.
- W1805174755 hasConcept C22744801 @default.
- W1805174755 hasConcept C2776256026 @default.
- W1805174755 hasConcept C2778297628 @default.
- W1805174755 hasConcept C39347974 @default.
- W1805174755 hasConcept C502942594 @default.
- W1805174755 hasConcept C54009773 @default.
- W1805174755 hasConcept C54355233 @default.
- W1805174755 hasConcept C553184892 @default.
- W1805174755 hasConcept C55493867 @default.
- W1805174755 hasConcept C71924100 @default.
- W1805174755 hasConcept C81729549 @default.
- W1805174755 hasConcept C81885089 @default.
- W1805174755 hasConcept C86803240 @default.
- W1805174755 hasConcept C8891405 @default.
- W1805174755 hasConceptScore W1805174755C104317684 @default.
- W1805174755 hasConceptScore W1805174755C143998085 @default.
- W1805174755 hasConceptScore W1805174755C153911025 @default.
- W1805174755 hasConceptScore W1805174755C154317977 @default.
- W1805174755 hasConceptScore W1805174755C190283241 @default.
- W1805174755 hasConceptScore W1805174755C202751555 @default.
- W1805174755 hasConceptScore W1805174755C203014093 @default.
- W1805174755 hasConceptScore W1805174755C22744801 @default.
- W1805174755 hasConceptScore W1805174755C2776256026 @default.
- W1805174755 hasConceptScore W1805174755C2778297628 @default.
- W1805174755 hasConceptScore W1805174755C39347974 @default.
- W1805174755 hasConceptScore W1805174755C502942594 @default.
- W1805174755 hasConceptScore W1805174755C54009773 @default.
- W1805174755 hasConceptScore W1805174755C54355233 @default.
- W1805174755 hasConceptScore W1805174755C553184892 @default.
- W1805174755 hasConceptScore W1805174755C55493867 @default.
- W1805174755 hasConceptScore W1805174755C71924100 @default.
- W1805174755 hasConceptScore W1805174755C81729549 @default.
- W1805174755 hasConceptScore W1805174755C81885089 @default.
- W1805174755 hasConceptScore W1805174755C86803240 @default.
- W1805174755 hasConceptScore W1805174755C8891405 @default.
- W1805174755 hasLocation W18051747551 @default.
- W1805174755 hasOpenAccess W1805174755 @default.
- W1805174755 hasPrimaryLocation W18051747551 @default.
- W1805174755 hasRelatedWork W1867633421 @default.
- W1805174755 hasRelatedWork W2048982755 @default.
- W1805174755 hasRelatedWork W2069066487 @default.
- W1805174755 hasRelatedWork W2348719231 @default.
- W1805174755 hasRelatedWork W2349388752 @default.
- W1805174755 hasRelatedWork W2350682502 @default.
- W1805174755 hasRelatedWork W2364836168 @default.
- W1805174755 hasRelatedWork W2366814281 @default.
- W1805174755 hasRelatedWork W2368778033 @default.
- W1805174755 hasRelatedWork W2369199386 @default.
- W1805174755 hasRelatedWork W2371569694 @default.
- W1805174755 hasRelatedWork W2384585209 @default.
- W1805174755 hasRelatedWork W2392095311 @default.
- W1805174755 hasRelatedWork W2403428885 @default.
- W1805174755 hasRelatedWork W2408823547 @default.
- W1805174755 hasRelatedWork W2778590633 @default.
- W1805174755 hasRelatedWork W2999701992 @default.
- W1805174755 hasRelatedWork W3030171247 @default.
- W1805174755 hasRelatedWork W3029936274 @default.
- W1805174755 hasRelatedWork W3197267127 @default.
- W1805174755 hasVolume "42" @default.
- W1805174755 isParatext "false" @default.
- W1805174755 isRetracted "false" @default.
- W1805174755 magId "1805174755" @default.
- W1805174755 workType "article" @default.