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• W1805388170 abstract "Tissue culture technologies are an important aspect of the genetic modification of grapevine ( Vitis vinifera ). The molecular basis of this phenomenon is not well understood, however, the extent of DNA methylation is recognised as a factor in the control of gene expression. This study explores the possibility that DNA methylation may have a role in grapevine culture responses. DNA methylation profiles were constructed for Vitis vinifera , cv. Sultanina, during plantlet micropropagation, callus induction and proliferation. Methylation of genomic DNA and ribosomal RNA genes (rDNA) was found in glasshouse-grown plants, micropropagated plantlets and callus cultures. An analysis of rDNA showed that glasshouse-grown plants had 74.6% of the recognition sequences for Hpa II methylated at the internal cytosine position CmCGG, whereas 7.7% of the recognition sequences appeared to be methylated at the external cytosine (mCmCGG). The rDNA profiles of micropropagated subcultures S 0 (initial subcultures) and S 4 (fourth subcultures), representing one year of in vitro growth, showed that the percentage of recognition sequences containing a methylated external cytosine increased from 7.7% in glasshouse-grown plants to 64.5% for S-0 and 72.5% for the S 4 subculture. The implications of these findings for the in vitro manipulation of grapevine used in genetic modifications are discussed." @default.
• W1805388170 created "2016-06-24" @default.
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• W1805388170 date "2015-08-13" @default.
• W1805388170 modified "2023-09-26" @default.
• W1805388170 title "Methylated DNA changes associated with the initiation and maintenance of Vitis vinifera in vitro shoot and callus cultures: A possible mechanism for age-related changes" @default.
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