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- W180568470 abstract "In this study, restaurant waste was used as a substrate for the production of lactic acid andpolyhydroxyalkanoate (PHA) by Ralstonia eutropha (formerly known as Alcaligeneseutrophus). PHA production was achieved via a two-stage process; lactic acidproduction from restaurant waste followed by PHA production from lactic acid. In thefirst stage, the study was focused on optimization of culture condition for lactic acidproduction in batch anaerobic treatment. These treatments were carried out in a 2Lstirred-tank bioreactor with lL working volume. The pH was controlled using a pHcontroller by adding 3M NaOH automatically. The lactic acid produced was determinedby HPLC method. Optimization for best conditions of lactic acid production were doneon temperature, duration at controlled pH 7, seeding with appropriate inoculum andculture under sterile and non-sterile conditions. Lactic acid was dominantly produced(70-99%) during most of the anaerobic treatments followed by acetic (1-20%), butyric (0-8%) and propionic acid (0-1%). Among the three different temperatures investigated i.e.30°C, 37°C and 4SoC, 37°C was the best for lactic acid production (17 g/L). Studies on the effect of duration at controlled pH 7 showed that by controlling pH 7 for 12 hours,higher lactic acid was produced (40 g/L). Production of lactic acid was reduced when pH7 was controlled for longer period due to methanogenesis. Seeding with Lactobacillusrhamnosus results in higher production of lactic acid (67 g/L). Increased concentration oflactic acid was detected under sterile condition. In this study, the maximumconcentration of lactic acid produced from restaurant waste was 97.7 g/L when thetemperature was controlled at 37°C, controlled pH 7 throughout the experiment andseeded with L. rhamnosus under sterile condition.The supernatant collected from the treatment of highest lactic acid production wasused in the second stage for the production of polyhydroxyalkanoate. PHA was producedby Ralstonia eutropha strain ATCC 17699 in a 2L bioreactor (l L working volume) inbatch culture, using lactic acid as the sole carbon source during the production phase.The bacteria was first precultivated in a nutrient-rich medium for 24 h before inoculationinto the production medium i.e. treated restaurant waste containing 20-25 g/L lactic acidas the carbon source. Initial inoculum cell density was kept high at 4-5 g/L. Themaximum PHA concentration obtained after 70h was about 10 g/L, corresponding to 97% (gig) of cell dry weight." @default.
- W180568470 created "2016-06-24" @default.
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- W180568470 date "2001-01-01" @default.
- W180568470 modified "2023-09-27" @default.
- W180568470 title "Production of Lactic Acid and Polyhydroxyalkanoates (PHA) from Restaurant Waste" @default.
- W180568470 hasPublicationYear "2001" @default.
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