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• W180580495 abstract "Small-angle X-ray scattering (SAXS) is emerging as an important technique to characterize the structure of RNA molecules. While lower in resolution than X-ray crystallography or NMR spectroscopy, SAXS has the great advantage to have virtually no molecular weight limitations and does not require crystallization. In addition, SAXS can be readily applied under a large range of solution conditions, allowing to monitor RNA folding, ligand binding, and to characterize partially folded intermediates. Here, we review how the development of SAXS as a structural technique is driven by advances in computer algorithms that allow to reconstruct low-resolution electron density maps ab initio from scattering profiles. In addition, we delineate how these low-resolution models can be used in free energy electrostatics calculations. Finally, we discuss how one can exploit the hierarchical nature of RNA folding by combining the low resolution, global information provided by SAXS with local information on RNA structure, from either experiments or state-of-the-art RNA structure prediction algorithms, to further increase the resolution and quality of models obtained from SAXS." @default.
• W180580495 created "2016-06-24" @default.
• W180580495 creator A5010576393 @default.
• W180580495 creator A5039881572 @default.
• W180580495 date "2009-01-01" @default.
• W180580495 modified "2023-09-25" @default.
• W180580495 title "Use of Small Angle X-ray Scattering (SAXS) to Characterize Conformational States of Functional RNAs" @default.
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• W180580495 doi "https://doi.org/10.1016/s0076-6879(09)69011-x" @default.
• W180580495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20946792" @default.
• W180580495 hasPublicationYear "2009" @default.
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