FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1806304054> ?p ?o ?g. }

• W1806304054 endingPage "7" @default.
• W1806304054 startingPage "1521" @default.
• W1806304054 abstract "The expression of the c-myc gene has previously been shown to be elevated and deregulated in the human hepatoma cell line Hep G2 (B. E. Huber and S. S. Thorgeirsson, Cancer Res., 47: 3414-3420, 1987). We now report that the Hep G2 N-ras gene is activated to a dominant-acting, transforming gene by a missense mutation in codon 61. Hep G2 DNA produced transformed foci when transfected into NIH 3T3 cells. Subsequent to a secondary round of transfection, Southern blot analysis of tumorigenic NIH 3T3 foci demonstrated the presence of human N-ras sequences. Nucleotide sequence analysis of one Hep G2 N-ras allele demonstrated that codons 12, 13, and 59 were normal and that codon 61 had a missense mutation (CAA to CTA). This mutation results in the incorporation of leucine instead of glutamine at residue 61 of the N-ras gene product, p21. N-ras sequences were amplified by the polymerase chain reaction from both Hep G2 genomic DNA and Hep G2 complementary DNA. Analysis of the amplified sequences demonstrated that only one Hep G2 N-ras allele exhibited the codon 61 mutation and that both the mutant and normal alleles were transcribed. Northern blot analysis demonstrated equivalent steady-state levels of N-ras transcripts in Hep G2 cells and normal human liver. The steady-state levels of N-ras and ornithine decarboxylase transcripts were positively correlated suggesting a positive relationship between N-ras expression and the replication rate of Hep G2 cells. c-Ki-ras and c-Ha-ras transcripts were not detected in either Hep G2 cells or normal human liver. Immunoprecipitation experiments using the monoclonal antibody Y13-259 demonstrated the presence of p21 in Hep G2 cells. Expression of a dominant-acting, transforming N-ras gene, in conjunction with the altered regulation of the c-myc gene, documents two important genetic lesions that could be responsible for the transformed phenotype of Hep G2 cells." @default.
• W1806304054 created "2016-06-24" @default.
• W1806304054 creator A5020559816 @default.
• W1806304054 creator A5023183400 @default.
• W1806304054 creator A5084700266 @default.
• W1806304054 creator A5087862269 @default.
• W1806304054 date "1990-03-01" @default.
• W1806304054 modified "2023-09-23" @default.
• W1806304054 title "Characterization of a transforming N-ras gene in the human hepatoma cell line Hep G2: additional evidence for the importance of c-myc and ras cooperation in hepatocarcinogenesis." @default.
• W1806304054 cites W1492352384 @default.
• W1806304054 cites W1585617332 @default.
• W1806304054 cites W1616429637 @default.
• W1806304054 cites W1715102258 @default.
• W1806304054 cites W1823130351 @default.
• W1806304054 cites W1965058394 @default.
• W1806304054 cites W1969845182 @default.
• W1806304054 cites W1973091522 @default.
• W1806304054 cites W1977462710 @default.
• W1806304054 cites W1986409528 @default.
• W1806304054 cites W1989009382 @default.
• W1806304054 cites W1992409084 @default.
• W1806304054 cites W2000682752 @default.
• W1806304054 cites W2003969944 @default.
• W1806304054 cites W2004785803 @default.
• W1806304054 cites W2006405593 @default.
• W1806304054 cites W2009773967 @default.
• W1806304054 cites W2012850212 @default.
• W1806304054 cites W2027033860 @default.
• W1806304054 cites W2027807422 @default.
• W1806304054 cites W2030739003 @default.
• W1806304054 cites W2034248536 @default.
• W1806304054 cites W20371925 @default.
• W1806304054 cites W2039627575 @default.
• W1806304054 cites W2044014221 @default.
• W1806304054 cites W2051586659 @default.
• W1806304054 cites W2052997487 @default.
• W1806304054 cites W2053178532 @default.
• W1806304054 cites W2053813431 @default.
• W1806304054 cites W2054904912 @default.
• W1806304054 cites W2056527566 @default.
• W1806304054 cites W2072585523 @default.
• W1806304054 cites W2075468561 @default.
• W1806304054 cites W2082777785 @default.
• W1806304054 cites W2083690533 @default.
• W1806304054 cites W2089741493 @default.
• W1806304054 cites W2090118483 @default.
• W1806304054 cites W2099748016 @default.
• W1806304054 cites W2105324001 @default.
• W1806304054 cites W2106495814 @default.
• W1806304054 cites W2113210366 @default.
• W1806304054 cites W2121241773 @default.
• W1806304054 cites W2138270253 @default.
• W1806304054 cites W2141617559 @default.
• W1806304054 cites W2142466677 @default.
• W1806304054 cites W2148974436 @default.
• W1806304054 cites W2156787671 @default.
• W1806304054 cites W21751534 @default.
• W1806304054 cites W2257732560 @default.
• W1806304054 cites W2397292236 @default.
• W1806304054 cites W2409793681 @default.
• W1806304054 cites W2413423266 @default.
• W1806304054 cites W2419021202 @default.
• W1806304054 cites W2420466631 @default.
• W1806304054 cites W51836028 @default.
• W1806304054 cites W77825559 @default.
• W1806304054 cites W992746848 @default.
• W1806304054 cites W2467877061 @default.
• W1806304054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2154325" @default.
• W1806304054 hasPublicationYear "1990" @default.
• W1806304054 type Work @default.
• W1806304054 sameAs 1806304054 @default.
• W1806304054 citedByCount "11" @default.
• W1806304054 crossrefType "journal-article" @default.
• W1806304054 hasAuthorship W1806304054A5020559816 @default.
• W1806304054 hasAuthorship W1806304054A5023183400 @default.
• W1806304054 hasAuthorship W1806304054A5084700266 @default.
• W1806304054 hasAuthorship W1806304054A5087862269 @default.
• W1806304054 hasConcept C104317684 @default.
• W1806304054 hasConcept C133307689 @default.
• W1806304054 hasConcept C143065580 @default.
• W1806304054 hasConcept C150194340 @default.
• W1806304054 hasConcept C153911025 @default.
• W1806304054 hasConcept C177707886 @default.
• W1806304054 hasConcept C181674461 @default.
• W1806304054 hasConcept C501734568 @default.
• W1806304054 hasConcept C54009773 @default.
• W1806304054 hasConcept C54355233 @default.
• W1806304054 hasConcept C75563809 @default.
• W1806304054 hasConcept C81885089 @default.
• W1806304054 hasConcept C86803240 @default.
• W1806304054 hasConceptScore W1806304054C104317684 @default.
• W1806304054 hasConceptScore W1806304054C133307689 @default.
• W1806304054 hasConceptScore W1806304054C143065580 @default.
• W1806304054 hasConceptScore W1806304054C150194340 @default.
• W1806304054 hasConceptScore W1806304054C153911025 @default.
• W1806304054 hasConceptScore W1806304054C177707886 @default.
• W1806304054 hasConceptScore W1806304054C181674461 @default.
• W1806304054 hasConceptScore W1806304054C501734568 @default.

• next