Matches in SemOpenAlex for { <https://semopenalex.org/work/W1807029720> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1807029720 endingPage "1332" @default.
- W1807029720 startingPage "1324" @default.
- W1807029720 abstract "Neurospora crassa possesses multiple intracellular peptidases which display overlapping substrate specificities. They were readily detected by an in situ staining procedure for peptidases separated in polyacrylamide gels, within which the auxilliary enzyme, l -amino acid oxidase, was immobilized. Eleven different intracellular peptidases were identified by electrophoretic separation and verified by their individual patterns of substrate specificities. Most peptide substrates tested were hydrolyzed by several different peptidases. The multiple intracellular peptidases may play overlapping roles in several basic cell processes which involve peptidase activity. The amount of peptidase activity for leucylglycine present in crude extracts of cells grown under widely different conditions was relatively constant, suggesting that this enzyme may be constitutive, although alterations in the amounts of individual peptidase isozymes may occur. A single enzyme, designated peptidase II, was partially purified and obtained free from the other peptidase species. Peptidase II was found to be an aminopeptidase with activity toward many peptides of varied composition and size. It was more active with tripeptides than homologous dipeptides and showed strong activity toward methionine-containing peptides. This enzyme, with a molecular weight of about 37,000, was thermolabile at 65°C and was strongly inhibited by p -hydroxymercuribenzoate, Zn 2+ , Co 2+ , and Mn 2+ , but was insensitive to the serine protease inhibitor phenylmethylsulfonyl fluoride. Peptidase II apparently possesses an essential sulfhydryl group and may be a metalloenzyme." @default.
- W1807029720 created "2016-06-24" @default.
- W1807029720 creator A5025617323 @default.
- W1807029720 creator A5045338940 @default.
- W1807029720 date "1979-03-01" @default.
- W1807029720 modified "2023-09-27" @default.
- W1807029720 title "Multiple Intracellular Peptidases in Neurospora crassa" @default.
- W1807029720 cites W1499338991 @default.
- W1807029720 cites W1535853324 @default.
- W1807029720 cites W1581428301 @default.
- W1807029720 cites W1775749144 @default.
- W1807029720 cites W1865878751 @default.
- W1807029720 cites W1909239871 @default.
- W1807029720 cites W1965259745 @default.
- W1807029720 cites W1969076396 @default.
- W1807029720 cites W1971057631 @default.
- W1807029720 cites W2050283738 @default.
- W1807029720 cites W2078897233 @default.
- W1807029720 cites W2079832626 @default.
- W1807029720 cites W2109959995 @default.
- W1807029720 cites W2111928978 @default.
- W1807029720 cites W2158346377 @default.
- W1807029720 cites W4242580605 @default.
- W1807029720 cites W998956804 @default.
- W1807029720 doi "https://doi.org/10.1128/jb.137.3.1324-1332.1979" @default.
- W1807029720 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/218316" @default.
- W1807029720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/86534" @default.
- W1807029720 hasPublicationYear "1979" @default.
- W1807029720 type Work @default.
- W1807029720 sameAs 1807029720 @default.
- W1807029720 citedByCount "7" @default.
- W1807029720 crossrefType "journal-article" @default.
- W1807029720 hasAuthorship W1807029720A5025617323 @default.
- W1807029720 hasAuthorship W1807029720A5045338940 @default.
- W1807029720 hasBestOaLocation W18070297201 @default.
- W1807029720 hasConcept C104317684 @default.
- W1807029720 hasConcept C143065580 @default.
- W1807029720 hasConcept C167392928 @default.
- W1807029720 hasConcept C181199279 @default.
- W1807029720 hasConcept C2776580952 @default.
- W1807029720 hasConcept C2776714187 @default.
- W1807029720 hasConcept C2776739539 @default.
- W1807029720 hasConcept C2776769606 @default.
- W1807029720 hasConcept C2777807008 @default.
- W1807029720 hasConcept C2779281246 @default.
- W1807029720 hasConcept C2780985610 @default.
- W1807029720 hasConcept C515207424 @default.
- W1807029720 hasConcept C54374919 @default.
- W1807029720 hasConcept C55493867 @default.
- W1807029720 hasConcept C79879829 @default.
- W1807029720 hasConcept C80458899 @default.
- W1807029720 hasConcept C86803240 @default.
- W1807029720 hasConceptScore W1807029720C104317684 @default.
- W1807029720 hasConceptScore W1807029720C143065580 @default.
- W1807029720 hasConceptScore W1807029720C167392928 @default.
- W1807029720 hasConceptScore W1807029720C181199279 @default.
- W1807029720 hasConceptScore W1807029720C2776580952 @default.
- W1807029720 hasConceptScore W1807029720C2776714187 @default.
- W1807029720 hasConceptScore W1807029720C2776739539 @default.
- W1807029720 hasConceptScore W1807029720C2776769606 @default.
- W1807029720 hasConceptScore W1807029720C2777807008 @default.
- W1807029720 hasConceptScore W1807029720C2779281246 @default.
- W1807029720 hasConceptScore W1807029720C2780985610 @default.
- W1807029720 hasConceptScore W1807029720C515207424 @default.
- W1807029720 hasConceptScore W1807029720C54374919 @default.
- W1807029720 hasConceptScore W1807029720C55493867 @default.
- W1807029720 hasConceptScore W1807029720C79879829 @default.
- W1807029720 hasConceptScore W1807029720C80458899 @default.
- W1807029720 hasConceptScore W1807029720C86803240 @default.
- W1807029720 hasIssue "3" @default.
- W1807029720 hasLocation W18070297201 @default.
- W1807029720 hasLocation W18070297202 @default.
- W1807029720 hasLocation W18070297203 @default.
- W1807029720 hasOpenAccess W1807029720 @default.
- W1807029720 hasPrimaryLocation W18070297201 @default.
- W1807029720 hasRelatedWork W118954403 @default.
- W1807029720 hasRelatedWork W1581428301 @default.
- W1807029720 hasRelatedWork W1675997988 @default.
- W1807029720 hasRelatedWork W1807029720 @default.
- W1807029720 hasRelatedWork W1900331501 @default.
- W1807029720 hasRelatedWork W1988390977 @default.
- W1807029720 hasRelatedWork W2098457605 @default.
- W1807029720 hasRelatedWork W2477354332 @default.
- W1807029720 hasRelatedWork W2982787313 @default.
- W1807029720 hasRelatedWork W3010056926 @default.
- W1807029720 hasVolume "137" @default.
- W1807029720 isParatext "false" @default.
- W1807029720 isRetracted "false" @default.
- W1807029720 magId "1807029720" @default.
- W1807029720 workType "article" @default.