FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1807366173> ?p ?o ?g. }

• W1807366173 endingPage "336" @default.
• W1807366173 startingPage "333" @default.
• W1807366173 abstract "Gram-negative bacteria inhabit a broad range of ecological niches. For Escherichia coli, this includes river water as well as humans and animals, where it can be both a commensal and a pathogen. Intricate regulatory mechanisms ensure that bacteria have the right complement of β-barrel outer membrane proteins (OMPs) to enable adaptation to a particular habitat. Yet no mechanism is known for replacing OMPs in the outer membrane, an issue that is further confounded by the lack of an energy source and the high stability and abundance of OMPs. Here we uncover the process underpinning OMP turnover in E. coli and show it to be passive and binary in nature, in which old OMPs are displaced to the poles of growing cells as new OMPs take their place. Using fluorescent colicins as OMP-specific probes, in combination with ensemble and single-molecule fluorescence microscopy in vivo and in vitro, as well as molecular dynamics simulations, we established the mechanism for binary OMP partitioning. OMPs clustered to form ∼0.5-μm diameter islands, where their diffusion is restricted by promiscuous interactions with other OMPs. OMP islands were distributed throughout the cell and contained the Bam complex, which catalyses the insertion of OMPs in the outer membrane. However, OMP biogenesis occurred as a gradient that was highest at mid-cell but largely absent at cell poles. The cumulative effect is to push old OMP islands towards the poles of growing cells, leading to a binary distribution when cells divide. Hence, the outer membrane of a Gram-negative bacterium is a spatially and temporally organized structure, and this organization lies at the heart of how OMPs are turned over in the membrane." @default.
• W1807366173 created "2016-06-24" @default.
• W1807366173 creator A5007897202 @default.
• W1807366173 creator A5008393635 @default.
• W1807366173 creator A5008716907 @default.
• W1807366173 creator A5016957266 @default.
• W1807366173 creator A5021577519 @default.
• W1807366173 creator A5023347600 @default.
• W1807366173 creator A5025024523 @default.
• W1807366173 creator A5033107091 @default.
• W1807366173 creator A5041718990 @default.
• W1807366173 creator A5049764579 @default.
• W1807366173 creator A5058121725 @default.
• W1807366173 creator A5063979077 @default.
• W1807366173 creator A5075970914 @default.
• W1807366173 creator A5080383938 @default.
• W1807366173 creator A5082706216 @default.
• W1807366173 creator A5090005733 @default.
• W1807366173 date "2015-06-10" @default.
• W1807366173 modified "2023-09-27" @default.
• W1807366173 title "Supramolecular assemblies underpin turnover of outer membrane proteins in bacteria" @default.
• W1807366173 cites W1562725670 @default.
• W1807366173 cites W1785392081 @default.
• W1807366173 cites W1935369718 @default.
• W1807366173 cites W1971456069 @default.
• W1807366173 cites W1972963835 @default.
• W1807366173 cites W1978849431 @default.
• W1807366173 cites W1980310800 @default.
• W1807366173 cites W1980585256 @default.
• W1807366173 cites W1981483220 @default.
• W1807366173 cites W1983961421 @default.
• W1807366173 cites W1986203234 @default.
• W1807366173 cites W1992003720 @default.
• W1807366173 cites W2008023389 @default.
• W1807366173 cites W2009708167 @default.
• W1807366173 cites W2012727169 @default.
• W1807366173 cites W2016158361 @default.
• W1807366173 cites W2029667189 @default.
• W1807366173 cites W2031772330 @default.
• W1807366173 cites W2035505213 @default.
• W1807366173 cites W2041654055 @default.
• W1807366173 cites W2044307316 @default.
• W1807366173 cites W2044548799 @default.
• W1807366173 cites W2048614879 @default.
• W1807366173 cites W2058593618 @default.
• W1807366173 cites W2060097208 @default.
• W1807366173 cites W2060719766 @default.
• W1807366173 cites W2069519711 @default.
• W1807366173 cites W2070978351 @default.
• W1807366173 cites W2072252061 @default.
• W1807366173 cites W2073603853 @default.
• W1807366173 cites W2078286807 @default.
• W1807366173 cites W2082926712 @default.
• W1807366173 cites W2090363184 @default.
• W1807366173 cites W2090827852 @default.
• W1807366173 cites W2099540110 @default.
• W1807366173 cites W2100483890 @default.
• W1807366173 cites W2111967267 @default.
• W1807366173 cites W2115986304 @default.
• W1807366173 cites W2119267663 @default.
• W1807366173 cites W2120061656 @default.
• W1807366173 cites W2124778528 @default.
• W1807366173 cites W2124813297 @default.
• W1807366173 cites W2128267600 @default.
• W1807366173 cites W2132218454 @default.
• W1807366173 cites W2133152400 @default.
• W1807366173 cites W2133297096 @default.
• W1807366173 cites W2148708097 @default.
• W1807366173 cites W2149325862 @default.
• W1807366173 cites W2150881786 @default.
• W1807366173 cites W2152967503 @default.
• W1807366173 cites W2154282828 @default.
• W1807366173 cites W2155957331 @default.
• W1807366173 cites W2163388241 @default.
• W1807366173 cites W2167819403 @default.
• W1807366173 cites W2169242133 @default.
• W1807366173 cites W2170711116 @default.
• W1807366173 cites W2326239820 @default.
• W1807366173 doi "https://doi.org/10.1038/nature14461" @default.
• W1807366173 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4905513" @default.
• W1807366173 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26061769" @default.
• W1807366173 hasPublicationYear "2015" @default.
• W1807366173 type Work @default.
• W1807366173 sameAs 1807366173 @default.
• W1807366173 citedByCount "161" @default.
• W1807366173 countsByYear W18073661732015 @default.
• W1807366173 countsByYear W18073661732016 @default.
• W1807366173 countsByYear W18073661732017 @default.
• W1807366173 countsByYear W18073661732018 @default.
• W1807366173 countsByYear W18073661732019 @default.
• W1807366173 countsByYear W18073661732020 @default.
• W1807366173 countsByYear W18073661732021 @default.
• W1807366173 countsByYear W18073661732022 @default.
• W1807366173 countsByYear W18073661732023 @default.
• W1807366173 crossrefType "journal-article" @default.
• W1807366173 hasAuthorship W1807366173A5007897202 @default.
• W1807366173 hasAuthorship W1807366173A5008393635 @default.
• W1807366173 hasAuthorship W1807366173A5008716907 @default.

• next