FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1807702939> ?p ?o ?g. }

• W1807702939 endingPage "508" @default.
• W1807702939 startingPage "501" @default.
• W1807702939 abstract "Cell-based therapies to treat loss-of-function hormonal disorders such as diabetes and Parkinson's disease are routinely coupled with encapsulation strategies, but an understanding of when and why grafts fail in vivo is lacking. Consequently, investigators cannot clearly define the key factors that influence graft success. Although bioluminescence is a popular method to track the survival of free cells transplanted in preclinical models, little is known of the ability to use bioluminescence for real-time tracking of microencapsulated cells. Furthermore, the impact that dynamic imaging distances may have, due to freely-floating microcapsules in vivo, on cell survival monitoring is unknown. This work addresses these questions by applying bioluminescence to a pancreatic substitute based on microencapsulated cells. Recombinant insulin-secreting cells were transduced with a luciferase lentivirus and microencapsulated in Ba2+ crosslinked alginate for in vitro and in vivo studies. In vitro quantitative bioluminescence monitoring was possible and viable microencapsulated cells were followed in real time under both normoxic and anoxic conditions. Although in vivo dispersion of freely-floating microcapsules in the peritoneal cavity limited the analysis to a qualitative bioluminescence evaluation, signals consistently four orders of magnitude above background were clear indicators of temporal cell survival. Strong agreement between in vivo and in vitro cell proliferation over time was discovered by making direct bioluminescence comparisons between explanted microcapsules and parallel in vitro cultures. Broader application of this bioluminescence approach to retrievable transplants, in supplement to currently used end-point physiological tests, could improve understanding and accelerate development of cell-based therapies for critical clinical applications. Copyright © 2014 John Wiley & Sons, Ltd." @default.
• W1807702939 created "2016-06-24" @default.
• W1807702939 creator A5049533278 @default.
• W1807702939 creator A5085660598 @default.
• W1807702939 date "2014-07-22" @default.
• W1807702939 modified "2023-09-24" @default.
• W1807702939 title "Bioluminescence tracking of alginate micro-encapsulated cell transplants" @default.
• W1807702939 cites W1963573978 @default.
• W1807702939 cites W1975257405 @default.
• W1807702939 cites W1977793698 @default.
• W1807702939 cites W1982197561 @default.
• W1807702939 cites W1983049414 @default.
• W1807702939 cites W1983711876 @default.
• W1807702939 cites W1985284905 @default.
• W1807702939 cites W1987623105 @default.
• W1807702939 cites W1999237160 @default.
• W1807702939 cites W2029183800 @default.
• W1807702939 cites W2036812817 @default.
• W1807702939 cites W2041376474 @default.
• W1807702939 cites W2050355889 @default.
• W1807702939 cites W2053234019 @default.
• W1807702939 cites W2053353941 @default.
• W1807702939 cites W2053387931 @default.
• W1807702939 cites W2062346334 @default.
• W1807702939 cites W2069544727 @default.
• W1807702939 cites W2076365503 @default.
• W1807702939 cites W2076766966 @default.
• W1807702939 cites W2077712899 @default.
• W1807702939 cites W2080277182 @default.
• W1807702939 cites W2088590107 @default.
• W1807702939 cites W2093130113 @default.
• W1807702939 cites W2093862830 @default.
• W1807702939 cites W2097132549 @default.
• W1807702939 cites W2097839388 @default.
• W1807702939 cites W2107989359 @default.
• W1807702939 cites W2115103319 @default.
• W1807702939 cites W2115317718 @default.
• W1807702939 cites W2146694099 @default.
• W1807702939 cites W2148618628 @default.
• W1807702939 cites W2151367313 @default.
• W1807702939 cites W2333211562 @default.
• W1807702939 doi "https://doi.org/10.1002/term.1946" @default.
• W1807702939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25047413" @default.
• W1807702939 hasPublicationYear "2014" @default.
• W1807702939 type Work @default.
• W1807702939 sameAs 1807702939 @default.
• W1807702939 citedByCount "2" @default.
• W1807702939 countsByYear W18077029392019 @default.
• W1807702939 countsByYear W18077029392022 @default.
• W1807702939 crossrefType "journal-article" @default.
• W1807702939 hasAuthorship W1807702939A5049533278 @default.
• W1807702939 hasAuthorship W1807702939A5085660598 @default.
• W1807702939 hasBestOaLocation W18077029391 @default.
• W1807702939 hasConcept C111335760 @default.
• W1807702939 hasConcept C136229726 @default.
• W1807702939 hasConcept C1491633281 @default.
• W1807702939 hasConcept C150903083 @default.
• W1807702939 hasConcept C161997846 @default.
• W1807702939 hasConcept C185592680 @default.
• W1807702939 hasConcept C202751555 @default.
• W1807702939 hasConcept C207001950 @default.
• W1807702939 hasConcept C5176359 @default.
• W1807702939 hasConcept C54009773 @default.
• W1807702939 hasConcept C54355233 @default.
• W1807702939 hasConcept C55493867 @default.
• W1807702939 hasConcept C71924100 @default.
• W1807702939 hasConcept C81885089 @default.
• W1807702939 hasConcept C86803240 @default.
• W1807702939 hasConcept C95444343 @default.
• W1807702939 hasConceptScore W1807702939C111335760 @default.
• W1807702939 hasConceptScore W1807702939C136229726 @default.
• W1807702939 hasConceptScore W1807702939C1491633281 @default.
• W1807702939 hasConceptScore W1807702939C150903083 @default.
• W1807702939 hasConceptScore W1807702939C161997846 @default.
• W1807702939 hasConceptScore W1807702939C185592680 @default.
• W1807702939 hasConceptScore W1807702939C202751555 @default.
• W1807702939 hasConceptScore W1807702939C207001950 @default.
• W1807702939 hasConceptScore W1807702939C5176359 @default.
• W1807702939 hasConceptScore W1807702939C54009773 @default.
• W1807702939 hasConceptScore W1807702939C54355233 @default.
• W1807702939 hasConceptScore W1807702939C55493867 @default.
• W1807702939 hasConceptScore W1807702939C71924100 @default.
• W1807702939 hasConceptScore W1807702939C81885089 @default.
• W1807702939 hasConceptScore W1807702939C86803240 @default.
• W1807702939 hasConceptScore W1807702939C95444343 @default.
• W1807702939 hasFunder F4320332161 @default.
• W1807702939 hasIssue "2" @default.
• W1807702939 hasLocation W18077029391 @default.
• W1807702939 hasLocation W18077029392 @default.
• W1807702939 hasOpenAccess W1807702939 @default.
• W1807702939 hasPrimaryLocation W18077029391 @default.
• W1807702939 hasRelatedWork W1964907928 @default.
• W1807702939 hasRelatedWork W2116391778 @default.
• W1807702939 hasRelatedWork W2357151295 @default.
• W1807702939 hasRelatedWork W2524058538 @default.
• W1807702939 hasRelatedWork W2626043046 @default.
• W1807702939 hasRelatedWork W2751024554 @default.
• W1807702939 hasRelatedWork W2939445688 @default.

• next