FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1810073570> ?p ?o ?g. }

• W1810073570 endingPage "398" @default.
• W1810073570 startingPage "385" @default.
• W1810073570 abstract "Cells of the arterial wall including macrophages were shown to oxidize low density lipoprotein (LDL) in vitro. Upon incubation of LDL with J-774.A1 macrophage-like cell line for 18 h in the presence of 1 microM CuSO4, extensive macrophage-mediated oxidation of the LDL fatty acids and cholesterol moieties was demonstrated. Similar results were found with mouse peritoneal macrophages or human monocyte-derived macrophages. Several lines of evidence suggest that LDL binding to the LDL receptor on macrophages is required for the cell-mediated oxidation of LDL. 1) Incubation of the cells in the presence of monoclonal antibody to the LDL receptor (IgG-C7), substantially inhibited lipoprotein oxidation. 2) Pretreatment of LDL with monoclonal antibodies to the LDL receptor binding domains on the LDL apoB-100 (mAbs B1B6 and B1B3) inhibited cell-mediated oxidation of LDL by 52-95%. 3) Down-regulation of the macrophage LDL receptors (by preloading the cells with cholesterol) reduced LDL oxidation by 42%. 4) Up-regulation of the LDL receptor (by macrophage incubation in serum-free medium) was associated with 80% elevation in LDL oxidation. 5) Macrophage activation with lipopolysaccharide up-regulated the LDL receptors and was associated with up twofold increase LDL oxidation. 6) Human monocyte-derived macrophages from a patient with homozygous familial hypercholesterolemia, which lack the LDL receptor, failed to oxidize the LDL. 7) On using acetylated LDL or methylated LDL, which do not bind to the LDL receptor, macrophage-mediated oxidation of the lipoprotein did not occur. The binding of LDL to the macrophage LDL receptor under oxidative stress induced the oxidation of extracellular unbound LDL as demonstrated by cell-mediated lipid peroxidation of mAb B1B6-treated LDL by cells that were preincubated with native LDL. Furthermore, macrophage conditioned medium (MCM) that was obtained after 5 h of cells preincubation with native LDL under oxidative stress (1 microM CuSO4), followed by lipoprotein removal and a further 18 h of cell incubation (but not MCM that was similarly obtained without cell preincubation with LDL), was found to contain oxidized linoleic and arachidonic acids and was able to induce LDL lipids peroxidation. In conclusion, macrophage-mediated oxidation of LDL requires an initial binding of the lipoprotein to the LDL receptor on the cell surface under oxidative stress. This interaction leads to the formation and release of cellular oxidized polyunsaturated fatty acids that can oxidize the LDL molecule extracellularly." @default.
• W1810073570 created "2016-06-24" @default.
• W1810073570 creator A5052487247 @default.
• W1810073570 creator A5082462956 @default.
• W1810073570 date "1994-03-01" @default.
• W1810073570 modified "2023-10-18" @default.
• W1810073570 title "Macrophage-mediated oxidation of extracellular low density lipoprotein requires an initial binding of the lipoprotein to its receptor." @default.
• W1810073570 cites W1510948177 @default.
• W1810073570 cites W1513897103 @default.
• W1810073570 cites W1539545968 @default.
• W1810073570 cites W1550297117 @default.
• W1810073570 cites W1562522900 @default.
• W1810073570 cites W1590457525 @default.
• W1810073570 cites W1597723737 @default.
• W1810073570 cites W1606357879 @default.
• W1810073570 cites W1673586429 @default.
• W1810073570 cites W176599066 @default.
• W1810073570 cites W1775749144 @default.
• W1810073570 cites W1841006369 @default.
• W1810073570 cites W1857052429 @default.
• W1810073570 cites W1964651215 @default.
• W1810073570 cites W1968567802 @default.
• W1810073570 cites W1973432977 @default.
• W1810073570 cites W1977093640 @default.
• W1810073570 cites W1978383066 @default.
• W1810073570 cites W1978919745 @default.
• W1810073570 cites W1979655335 @default.
• W1810073570 cites W1984393490 @default.
• W1810073570 cites W1985929579 @default.
• W1810073570 cites W1990468555 @default.
• W1810073570 cites W1991827124 @default.
• W1810073570 cites W1998043006 @default.
• W1810073570 cites W2004789344 @default.
• W1810073570 cites W2005182309 @default.
• W1810073570 cites W2005230846 @default.
• W1810073570 cites W2006200791 @default.
• W1810073570 cites W2011390446 @default.
• W1810073570 cites W2020794109 @default.
• W1810073570 cites W2032731208 @default.
• W1810073570 cites W2033684021 @default.
• W1810073570 cites W2035771062 @default.
• W1810073570 cites W2038213399 @default.
• W1810073570 cites W2041504897 @default.
• W1810073570 cites W2044900626 @default.
• W1810073570 cites W2045482735 @default.
• W1810073570 cites W2059814953 @default.
• W1810073570 cites W2067772335 @default.
• W1810073570 cites W2068053744 @default.
• W1810073570 cites W2073000116 @default.
• W1810073570 cites W2076332509 @default.
• W1810073570 cites W2076671707 @default.
• W1810073570 cites W2076945762 @default.
• W1810073570 cites W2077938509 @default.
• W1810073570 cites W2079614608 @default.
• W1810073570 cites W2079984226 @default.
• W1810073570 cites W2081304820 @default.
• W1810073570 cites W2089354332 @default.
• W1810073570 cites W2095840354 @default.
• W1810073570 cites W2113553969 @default.
• W1810073570 cites W2113620629 @default.
• W1810073570 cites W2114462793 @default.
• W1810073570 cites W2120626817 @default.
• W1810073570 cites W2138257090 @default.
• W1810073570 cites W2140328938 @default.
• W1810073570 cites W2144230929 @default.
• W1810073570 cites W2153748987 @default.
• W1810073570 cites W2154760942 @default.
• W1810073570 cites W2155810202 @default.
• W1810073570 cites W2161491104 @default.
• W1810073570 cites W2162458714 @default.
• W1810073570 cites W2166970235 @default.
• W1810073570 cites W2253540608 @default.
• W1810073570 cites W3022864527 @default.
• W1810073570 cites W3139808032 @default.
• W1810073570 cites W40251019 @default.
• W1810073570 cites W4376600567 @default.
• W1810073570 doi "https://doi.org/10.1016/s0022-2275(20)41191-5" @default.
• W1810073570 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8014575" @default.
• W1810073570 hasPublicationYear "1994" @default.
• W1810073570 type Work @default.
• W1810073570 sameAs 1810073570 @default.
• W1810073570 citedByCount "79" @default.
• W1810073570 countsByYear W18100735702012 @default.
• W1810073570 countsByYear W18100735702013 @default.
• W1810073570 countsByYear W18100735702016 @default.
• W1810073570 countsByYear W18100735702019 @default.
• W1810073570 countsByYear W18100735702023 @default.
• W1810073570 crossrefType "journal-article" @default.
• W1810073570 hasAuthorship W1810073570A5052487247 @default.
• W1810073570 hasAuthorship W1810073570A5082462956 @default.
• W1810073570 hasBestOaLocation W18100735701 @default.
• W1810073570 hasConcept C170493617 @default.
• W1810073570 hasConcept C185592680 @default.
• W1810073570 hasConcept C202751555 @default.
• W1810073570 hasConcept C2778163477 @default.
• W1810073570 hasConcept C2779244956 @default.
• W1810073570 hasConcept C2779620165 @default.
• W1810073570 hasConcept C2780072125 @default.

• next