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- W181027309 abstract "Amperometric microelectrode sensors were fabricated with the length and area of the electroactive surface tailored for the dimensions of the cells under investigation. A procedure for producing a sharpened carbon fiber sensor substrate was developed in order to avoid cell membrane rupture during single cell implantation. This carbon fiber sensor was then coated with a conductive polymeric porphyrin capable of the selective measurement of a specific metal ion. Differential pulse voltammetry was used for the determination of the concentration of the metal ions on the coated surface of the sensor. Tris(4-N-methylpyridyl)p-hydroxyphenylporphyrin was deposited on the surface of a carbon fiber ultramicroelectrode to measure Ca{sup 2+} concentration of 1 {times} 10{sup {minus}5} M in BHK-2/C-13 cells. Ni{sup 2+} at 1 {times} 10{sup {minus}6} M were measured in individual BC3H-T-myocytes, H4-IIC3 rat hepatoma or BHK-2/C-13 cells using ultramicroelectrodes coated with 3-methoxy-4-hydroxy-phenylporphyrin. In conclusion, these ultramicroelectrodes are capable of measuring the concentration of unbound Ni{sup 2+} and Ca{sup 2+} in the pathophysiologic range. The porphyrin ring may be modified by attaching different substituents with specificity for the coordination other metal cations." @default.
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- W181027309 date "1991-03-15" @default.
- W181027309 modified "2023-10-16" @default.
- W181027309 title "Amperometric microsensors for metal ion determination in single biological cells" @default.
- W181027309 hasPublicationYear "1991" @default.
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