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- W1810274712 abstract "int-2 is one of two cellular genes (int-1 and int-2) currently implicated in the genesis of mammary carcinomas by mouse mammary tumor virus and may constitute a novel cellular proto-oncogene. Using low-stringency hybridization with mouse int-2 probes, we established that homologous genes exist in a variety of mammalian species, including humans, but failed to detect related sequences in other classes and phyla. Recombinant bacteriophage clones and a single cosmid encompassing the human int-2 gene were isolated and characterized by restriction enzyme mapping. A survey of nine primary human breast tumors, three breast tumor cell lines, and three normal individuals revealed no evidence for gross amplification or rearrangement of the int-2 locus. Three distinct restriction fragment length polymorphisms were observed which could prove useful in future linkage studies. By a combination of in situ hybridization of metaphase chromosomes and somatic cell genetics, the human int-2 gene was mapped to chromosome 11, band q13." @default.
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- W1810274712 date "1986-02-01" @default.
- W1810274712 modified "2023-09-27" @default.
- W1810274712 title "Characterization and Chromosome Assignment of the Human Homolog of <i>int-2</i>, a Potential Proto-Oncogene" @default.
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- W1810274712 doi "https://doi.org/10.1128/mcb.6.2.502-510.1986" @default.
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