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- W181143942 abstract "Endogenous oxygen-18(/sup 18/O) content in the rat brain was determined as the /sup 18/F image by means of the nuclear reaction and the autoradiography (ARG). Endogenous /sup 18/O which contained 0.204% in total oxygen was converted to /sup 18/F by the /sup 18/O (p,n) /sup 18/F reaction. The rat brain slice(34..mu..m), the rat brain homogenate slices(34..mu..m) contained various percent of water (65,75 and 85%), and 24..mu..m polyester film (33% of oxygen) as a /sup 18/O standard were placed on the aluminum target holder cooled to -100/sup 0/C by liquid N/sub 2/. Proton beam(18MeV) was scattered and decayed to 5.35MeV and Ti, Al foils, He gas and 100 ..mu..m polyester film to irradiate the target. The slices were irradiated for 30 min at 15 ..mu..A to need the enough activity for ARG. Uniformity of the beam density was also measured by 100 ..mu..m polyester film set to precede the target. Quantitative ARG of the slices were processed 3 hours after the irradiation to decay out the other radio-activities (/sup 11/C and /sup 13/N). /sup 18/F image which consists of both H/sub 2//sup 18/O and /sup 18/O in non-water materials in the rat brain was visualized. The ARG density and the oxygenmore » content showed their linear relationship. This method will be useful to apply to the various brain studies (e.g. CBF study, water content in brain edema and the metabolism of /sup 18/O labeled compounds.)« less" @default.
- W181143942 created "2016-06-24" @default.
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- W181143942 date "1984-01-01" @default.
- W181143942 modified "2023-09-22" @default.
- W181143942 title "Activation autoradiography: A new method for the determination of endogenous oxygen-18" @default.
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