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• W1811814528 endingPage "1902" @default.
• W1811814528 startingPage "1893" @default.
• W1811814528 abstract "Blue native PAGE (BN‐PAGE) is a powerful method to separate protein complexes while preserving their native state. However, the resolution of the method is limited as complexes with similar molecular masses cannot be resolved. Here we describe native 2DE using immobilized pH‐gradients in combination with BN‐PAGE to resolve protein complexes by their p I and molecular mass. This method enables electrophoretic separation of proteins between p I 3 and 10 and can resolve molecular masses up to 1.2 MDa. Visualized gel spots at large molecular weight were identified using MS to confirm potential protein complexes. Several protein complexes could be identified, most prominent GroEL in complex with GroES, parts of the ribosomal machinery and membrane transport system. In summary, this method enables easy high‐resolution electrophoretic separation of protein complexes." @default.
• W1811814528 created "2016-06-24" @default.
• W1811814528 creator A5037651436 @default.
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• W1811814528 date "2014-04-16" @default.
• W1811814528 modified "2023-09-25" @default.
• W1811814528 title "High resolution two-dimensional electrophoresis of native proteins" @default.
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• W1811814528 doi "https://doi.org/10.1002/elps.201400060" @default.
• W1811814528 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24644151" @default.
• W1811814528 hasPublicationYear "2014" @default.
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