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- W1815055905 abstract "Electron microscopy (EM) is a method appropriate for examining details of the sizes and shapes of biological macromolecules and is particularly useful in studying isolated or reconstituted macromolecular assemblies. Small amounts of material (often < 1 µg) can be used and prepared in a state suitable for viewing in as short a time as several minutes. The basic procedure involves adsorption of the biomolecules onto a support film, followed by staining or shadowing of the preparation and viewing of the dehydrated molecules in vacuo in an electron beam. A resolution of 1 to 2 nm is routine with conventional microscopes. The basic procedure can be adapted to give information about sites of specific epitopes, location of newly synthesized components, internal structures, and atomic composition. Structures most suitable for EM analysis are nucleoprotein complexes, including ribosomes, spliceosomes, nucleosomes, and virus particles. DNA and RNA molecules are also suitable, and their lengths can be directly related to the number of base pairs or nucleotide residues determined biochemically. In general, it is difficult to visualize small proteins (<50 000 Da), but good detail can be obtained if they are isolated as multimeric complexes or if they can be induced to form filaments or crystalline arrays. A wide range of applications are available using EM techniques, including virus identification, mapping of hybridized regions in heteroduplexes, detailing of macromolecular interaction, and analysis of the organization of molecular components in replication, transcription, splicing, and translation complexes." @default.
- W1815055905 created "2016-06-24" @default.
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- W1815055905 date "2006-09-15" @default.
- W1815055905 modified "2023-10-16" @default.
- W1815055905 title "Electron Microscopy of Biomolecules" @default.
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- W1815055905 doi "https://doi.org/10.1002/3527600906.mcb.200300057" @default.
- W1815055905 hasPublicationYear "2006" @default.
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