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- W1817548902 endingPage "101" @default.
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- W1817548902 abstract "The physiological role of topoisomerase III is unclear for any organism. We show here that the removal of topoisomerase III in temperature sensitive topoisomerase IV mutants in Escherichia coli results in inviability at the permissive temperature. The removal of topoisomerase III has no effect on the accumulation of catenated intermediates of DNA replication, even when topoisomerase IV activity is removed. Either recQ or recA null mutations, but not helD null or lexA3, partially rescued the synthetic lethality of the double topoisomerase III/IV mutant, indicating a role for topoisomerase III in recombination. We find a bias against deleting the gene encoding topoisomerase III in ruvC53 or DeltaruvABC backgrounds compared with the isogenic wild-type strains. The topoisomerase III RuvC double mutants that can be constructed are five- to 10-fold more sensitive to UV irradiation and mitomycin C treatment and are twofold less efficient in transduction efficiency than ruvC53 mutants. The overexpression of ruvABC allows the construction of the topoisomerase III/IV double mutant. These data are consistent with a role for topoisomerase III in disentangling recombination intermediates as an alternative to RuvABC to maintain the stability of the genome." @default.
- W1817548902 created "2016-06-24" @default.
- W1817548902 creator A5006513406 @default.
- W1817548902 creator A5008354080 @default.
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- W1817548902 creator A5076354058 @default.
- W1817548902 creator A5080139631 @default.
- W1817548902 date "2005-09-05" @default.
- W1817548902 modified "2023-10-15" @default.
- W1817548902 title "A role for topoisomerase III in a recombination pathway alternative to RuvABC" @default.
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