Matches in SemOpenAlex for { <https://semopenalex.org/work/W1817751043> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1817751043 endingPage "52" @default.
- W1817751043 startingPage "47" @default.
- W1817751043 abstract "We have identified a factor from rat liver cytosol that enhances the DNA-cellulose-binding ability of the glucocorticoid receptor and lowers the sedimentation value from 9-10 S to 4-5 S. Cytosol is prepared in the presence of molybdate, and unactivated receptor is isolated by chromatography on DEAE-cellulose in the presence of molybdate. This receptor sediments at 9-10 S and has little affinity for DNA. If the molybdate is removed and the receptor is incubated at 25 degrees C with the low-salt wash of the DEAE-cellulose column, DNA binding is enhanced by 50-600% relative to controls incubated with buffer only. In addition, the factor present in the low-salt wash converts the 9-10 S receptor into a mixture of 5 S and 4 S forms. The factor must be present during the incubation in order to exert its maximal effect. Factor added after the incubation has only marginal effects on the DNA-binding ability of the receptor, indicating that the factor does not increase the DNA-binding ability of activated receptor. Moreover, the factor is significantly less effective on receptor that has been activated before incubation with the factor. These results suggest that the factor acts as an activation enhancer. Preliminary characterization indicates that the activation enhancer is a trypsin-sensitive protein of approx. 70,000 Da, whose activation-enhancing properties are inhibited by ATP. RNAase A, which has effects similar to those described above on the 7-8 S receptor, does not mimic the effects of the activation enhancer on the 9-10 S receptor." @default.
- W1817751043 created "2016-06-24" @default.
- W1817751043 creator A5006995542 @default.
- W1817751043 creator A5029863008 @default.
- W1817751043 creator A5030415662 @default.
- W1817751043 creator A5077889849 @default.
- W1817751043 creator A5088281177 @default.
- W1817751043 date "1988-11-15" @default.
- W1817751043 modified "2023-09-27" @default.
- W1817751043 title "Identification of a protein from rat liver cytosol that enhances activation of the glucocorticoid receptor" @default.
- W1817751043 cites W1516283219 @default.
- W1817751043 cites W1546675367 @default.
- W1817751043 cites W1574229173 @default.
- W1817751043 cites W1881700477 @default.
- W1817751043 cites W1973798024 @default.
- W1817751043 cites W1977093640 @default.
- W1817751043 cites W1977613095 @default.
- W1817751043 cites W1981918631 @default.
- W1817751043 cites W2009435038 @default.
- W1817751043 cites W2011656910 @default.
- W1817751043 cites W2014139892 @default.
- W1817751043 cites W2018531293 @default.
- W1817751043 cites W2021651508 @default.
- W1817751043 cites W2022411824 @default.
- W1817751043 cites W2033014787 @default.
- W1817751043 cites W2058941919 @default.
- W1817751043 cites W2070255166 @default.
- W1817751043 cites W2074076851 @default.
- W1817751043 cites W2303503630 @default.
- W1817751043 cites W1983172509 @default.
- W1817751043 doi "https://doi.org/10.1042/bj2560047" @default.
- W1817751043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1135366" @default.
- W1817751043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3223910" @default.
- W1817751043 hasPublicationYear "1988" @default.
- W1817751043 type Work @default.
- W1817751043 sameAs 1817751043 @default.
- W1817751043 citedByCount "5" @default.
- W1817751043 crossrefType "journal-article" @default.
- W1817751043 hasAuthorship W1817751043A5006995542 @default.
- W1817751043 hasAuthorship W1817751043A5029863008 @default.
- W1817751043 hasAuthorship W1817751043A5030415662 @default.
- W1817751043 hasAuthorship W1817751043A5077889849 @default.
- W1817751043 hasAuthorship W1817751043A5088281177 @default.
- W1817751043 hasBestOaLocation W18177510432 @default.
- W1817751043 hasConcept C104317684 @default.
- W1817751043 hasConcept C111936080 @default.
- W1817751043 hasConcept C153911025 @default.
- W1817751043 hasConcept C170493617 @default.
- W1817751043 hasConcept C178790620 @default.
- W1817751043 hasConcept C181199279 @default.
- W1817751043 hasConcept C185592680 @default.
- W1817751043 hasConcept C25642318 @default.
- W1817751043 hasConcept C2775981058 @default.
- W1817751043 hasConcept C2780340462 @default.
- W1817751043 hasConcept C55493867 @default.
- W1817751043 hasConcept C59493245 @default.
- W1817751043 hasConcept C86339819 @default.
- W1817751043 hasConcept C86803240 @default.
- W1817751043 hasConcept C98539663 @default.
- W1817751043 hasConceptScore W1817751043C104317684 @default.
- W1817751043 hasConceptScore W1817751043C111936080 @default.
- W1817751043 hasConceptScore W1817751043C153911025 @default.
- W1817751043 hasConceptScore W1817751043C170493617 @default.
- W1817751043 hasConceptScore W1817751043C178790620 @default.
- W1817751043 hasConceptScore W1817751043C181199279 @default.
- W1817751043 hasConceptScore W1817751043C185592680 @default.
- W1817751043 hasConceptScore W1817751043C25642318 @default.
- W1817751043 hasConceptScore W1817751043C2775981058 @default.
- W1817751043 hasConceptScore W1817751043C2780340462 @default.
- W1817751043 hasConceptScore W1817751043C55493867 @default.
- W1817751043 hasConceptScore W1817751043C59493245 @default.
- W1817751043 hasConceptScore W1817751043C86339819 @default.
- W1817751043 hasConceptScore W1817751043C86803240 @default.
- W1817751043 hasConceptScore W1817751043C98539663 @default.
- W1817751043 hasIssue "1" @default.
- W1817751043 hasLocation W18177510431 @default.
- W1817751043 hasLocation W18177510432 @default.
- W1817751043 hasLocation W18177510433 @default.
- W1817751043 hasLocation W18177510434 @default.
- W1817751043 hasOpenAccess W1817751043 @default.
- W1817751043 hasPrimaryLocation W18177510431 @default.
- W1817751043 hasRelatedWork W1801699123 @default.
- W1817751043 hasRelatedWork W1972701187 @default.
- W1817751043 hasRelatedWork W2029470650 @default.
- W1817751043 hasRelatedWork W2055670227 @default.
- W1817751043 hasRelatedWork W2080189603 @default.
- W1817751043 hasRelatedWork W2083816668 @default.
- W1817751043 hasRelatedWork W2092395624 @default.
- W1817751043 hasRelatedWork W2114256457 @default.
- W1817751043 hasRelatedWork W2211434907 @default.
- W1817751043 hasRelatedWork W2433748563 @default.
- W1817751043 hasVolume "256" @default.
- W1817751043 isParatext "false" @default.
- W1817751043 isRetracted "false" @default.
- W1817751043 magId "1817751043" @default.
- W1817751043 workType "article" @default.