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- W1817811857 abstract "A simple procedure for the concentration and partial purification of enteroviruses from tissue culture harvests is described. After removal of acid-precipitating components with a cationic detergent, the detergent and most membrane-coating components were removed by treatment with a cationic-exchange resin. The resin effluent was then acidified, and the virus was adsorbed to epoxy-fiberglass membranes. Virus was then eluted with pH 11.5 glycine-NaOH buffer. Since this eluate contains no orgcentrated simply by acidifying the eluate and passing it through a smaller membrane than that used for the first concentration. As high as 500-fold concentrations can be achieved, with a high efficiency of recovery." @default.
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- W1817811857 date "1976-11-01" @default.
- W1817811857 modified "2023-09-26" @default.
- W1817811857 title "Concentration and purification of enteroviruses by membrane chromatography" @default.
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- W1817811857 doi "https://doi.org/10.1128/aem.32.5.689-693.1976" @default.
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