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• W1817872890 endingPage "962" @default.
• W1817872890 startingPage "955" @default.
• W1817872890 abstract "A circular DNA plasmid, pSR1, isolated from Zygosaccharomyces rouxii has a pair of inverted repeats consisting of completely homologous 959-base pair (bp) sequences. Intramolecular recombination occurs frequently at the inverted repeats in cells of Saccharomyces cerevisiae, as well as in Z. rouxii, and is catalyzed by a protein encoded by the R gene of its own genome. The recombination is, however, independent of the RAD52 gene of the host genome. A site for initiation of the intramolecular recombination in the S. cerevisiae host was delimited into, at most, a 58-bp region in the inverted repeats by using mutant plasmids created by linker insertion. The 58-bp region contains a pair with 14-bp dyad symmetry separated by a 3-bp spacer sequence. The recombination initiated at this site was accompanied by a high frequency of gene conversion (3 to 50% of the plasmid clones examined). Heterogeneity created by the linker insertion or by a deletion (at most 153 bp so far tested) at any place on the inverted repeats was converted to a homologous combination by the gene conversion, even in the rad52-1 mutant host. A mechanism implying branch migration coupled with DNA replication is discussed." @default.
• W1817872890 created "2016-06-24" @default.
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• W1817872890 date "1988-02-01" @default.
• W1817872890 modified "2023-09-26" @default.
• W1817872890 title "Gene conversion associated with site-specific recombination in yeast plasmid pSR1" @default.
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• W1817872890 doi "https://doi.org/10.1128/mcb.8.2.955-962.1988" @default.
• W1817872890 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/363228" @default.
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