FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1820852428> ?p ?o ?g. }

• W1820852428 endingPage "4525" @default.
• W1820852428 startingPage "4516" @default.
• W1820852428 abstract "Molecular cloning techniques were used to isolate and characterize the DNA including and surrounding the CDC24 and PYK1 genes on the left arm of chromosome I of the yeast Saccharomyces cerevisiae. A plasmid that complemented a temperature-sensitive cdc24 mutation was isolated from a yeast genomic DNA library in a shuttle vector. Plasmids containing pyk1-complementing DNA were obtained from other investigators. Several lines of evidence (including one-step gene replacement experiments) demonstrated that the complementing plasmids contained the bona fide CDC24 and PYK1 genes. These sequences were then used to isolate additional DNA from chromosome I by probing a yeast genomic DNA library in a lambda vector. A total of 28 kilobases (kb) of contiguous DNA surrounding the CDC24 and PYK1 genes was isolated, and a restriction map was determined. Electron microscopy of R-loop-containing DNA and RNA blot hybridization analyses indicated that an 18-kb segment contained at least seven transcribed regions, only three of which corresponded to previously known genes (CDC24, PYK1, and CYC3). Southern blot hybridization experiments suggested that none of the genes in this region was duplicated elsewhere in the yeast genome. The centers of CDC24 and PYK1 were only approximately 7.5 kb apart, although the genetic map distance between them is approximately 13 centimorgans. As previous studies with S. cerevisiae have indicated that 1 centimorgan generally corresponds to approximately 3 kb, the region between CDC24 and PYK1 appears to undergo meiotic recombination at an unusually high frequency." @default.
• W1820852428 created "2016-06-24" @default.
• W1820852428 creator A5010372182 @default.
• W1820852428 creator A5015038830 @default.
• W1820852428 creator A5023758485 @default.
• W1820852428 creator A5075986364 @default.
• W1820852428 date "1986-12-01" @default.
• W1820852428 modified "2023-09-25" @default.
• W1820852428 title "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome." @default.
• W1820852428 cites W1433017100 @default.
• W1820852428 cites W1489317543 @default.
• W1820852428 cites W1519084876 @default.
• W1820852428 cites W1536696467 @default.
• W1820852428 cites W1537782690 @default.
• W1820852428 cites W1547483008 @default.
• W1820852428 cites W1662971183 @default.
• W1820852428 cites W168383662 @default.
• W1820852428 cites W1764847066 @default.
• W1820852428 cites W1770450199 @default.
• W1820852428 cites W1779157911 @default.
• W1820852428 cites W1781717284 @default.
• W1820852428 cites W1805013980 @default.
• W1820852428 cites W1837263056 @default.
• W1820852428 cites W1837697192 @default.
• W1820852428 cites W1853516231 @default.
• W1820852428 cites W1855498923 @default.
• W1820852428 cites W1866329086 @default.
• W1820852428 cites W1933222543 @default.
• W1820852428 cites W193661486 @default.
• W1820852428 cites W1939501721 @default.
• W1820852428 cites W1956581008 @default.
• W1820852428 cites W1964876650 @default.
• W1820852428 cites W1965486134 @default.
• W1820852428 cites W1968121029 @default.
• W1820852428 cites W1968916548 @default.
• W1820852428 cites W1975726016 @default.
• W1820852428 cites W1979564628 @default.
• W1820852428 cites W1985910275 @default.
• W1820852428 cites W1986210752 @default.
• W1820852428 cites W1987056309 @default.
• W1820852428 cites W1992042701 @default.
• W1820852428 cites W1995420892 @default.
• W1820852428 cites W1999432616 @default.
• W1820852428 cites W2004351979 @default.
• W1820852428 cites W2010197246 @default.
• W1820852428 cites W2013404388 @default.
• W1820852428 cites W2013428651 @default.
• W1820852428 cites W2016613744 @default.
• W1820852428 cites W2018082807 @default.
• W1820852428 cites W2022016707 @default.
• W1820852428 cites W2025675183 @default.
• W1820852428 cites W2026642300 @default.
• W1820852428 cites W2028782768 @default.
• W1820852428 cites W2029611498 @default.
• W1820852428 cites W2029987769 @default.
• W1820852428 cites W2035101804 @default.
• W1820852428 cites W2036823377 @default.
• W1820852428 cites W2038912552 @default.
• W1820852428 cites W2050611086 @default.
• W1820852428 cites W2056240197 @default.
• W1820852428 cites W2057385958 @default.
• W1820852428 cites W2059645566 @default.
• W1820852428 cites W2060096281 @default.
• W1820852428 cites W2063450941 @default.
• W1820852428 cites W2064320633 @default.
• W1820852428 cites W2068069796 @default.
• W1820852428 cites W2071419632 @default.
• W1820852428 cites W2077077811 @default.
• W1820852428 cites W2084742641 @default.
• W1820852428 cites W2086955288 @default.
• W1820852428 cites W2088400936 @default.
• W1820852428 cites W2102536188 @default.
• W1820852428 cites W2109773803 @default.
• W1820852428 cites W2120487459 @default.
• W1820852428 cites W2121562033 @default.
• W1820852428 cites W2134397471 @default.
• W1820852428 cites W2144634347 @default.
• W1820852428 cites W2161695812 @default.
• W1820852428 cites W2166028173 @default.
• W1820852428 cites W2226935353 @default.
• W1820852428 cites W2302556351 @default.
• W1820852428 cites W2896474271 @default.
• W1820852428 cites W3081785928 @default.
• W1820852428 doi "https://doi.org/10.1128/mcb.6.12.4516" @default.
• W1820852428 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/367236" @default.
• W1820852428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3540615" @default.
• W1820852428 hasPublicationYear "1986" @default.
• W1820852428 type Work @default.
• W1820852428 sameAs 1820852428 @default.
• W1820852428 citedByCount "58" @default.
• W1820852428 crossrefType "journal-article" @default.
• W1820852428 hasAuthorship W1820852428A5010372182 @default.
• W1820852428 hasAuthorship W1820852428A5015038830 @default.
• W1820852428 hasAuthorship W1820852428A5023758485 @default.
• W1820852428 hasAuthorship W1820852428A5075986364 @default.
• W1820852428 hasBestOaLocation W18208524282 @default.
• W1820852428 hasConcept C104317684 @default.
• W1820852428 hasConcept C122735190 @default.

• next