Matches in SemOpenAlex for { <https://semopenalex.org/work/W1821047819> ?p ?o ?g. }
- W1821047819 endingPage "5731" @default.
- W1821047819 startingPage "5724" @default.
- W1821047819 abstract "Porous volumetric capture elements in microfluidic sensors are advantageous compared to planar capture surfaces due to higher reaction site density and decreased diffusion lengths that can reduce detection limits and total assay time. However a mismatch in refractive indices between the capture matrix and fluid within the porous interstices results in scattering of incident, reflected, or emitted light, significantly reducing the signal for optical detection. Here we demonstrate that perfusion of an index-matching fluid within a porous matrix minimizes scattering, thus enhancing optical signal by enabling the entire capture element volume to be probed. Signal enhancement is demonstrated for both fluorescence and absorbance detection, using porous polymer monoliths in a silica capillary and packed beds of glass beads within thermoplastic microchannels, respectively. Fluorescence signal was improved by a factor of 3.5× when measuring emission from a fluorescent compound attached directly to the polymer monolith, and up to 2.6× for a rapid 10 min direct immunoassay. When combining index matching with a silver enhancement step, a detection limit of 0.1 ng mL(-1) human IgG and a 5 log dynamic range was achieved. The demonstrated technique provides a simple method for enhancing optical sensitivity for a wide range of assays, enabling the full benefits of porous detection elements in miniaturized analytical systems to be realized." @default.
- W1821047819 created "2016-06-24" @default.
- W1821047819 creator A5016293970 @default.
- W1821047819 creator A5023886935 @default.
- W1821047819 creator A5029703684 @default.
- W1821047819 creator A5059575527 @default.
- W1821047819 creator A5069852433 @default.
- W1821047819 creator A5088362530 @default.
- W1821047819 date "2015-01-01" @default.
- W1821047819 modified "2023-10-17" @default.
- W1821047819 title "Optical detection enhancement in porous volumetric microfluidic capture elements using refractive index matching fluids" @default.
- W1821047819 cites W1965009947 @default.
- W1821047819 cites W1973333651 @default.
- W1821047819 cites W1973384073 @default.
- W1821047819 cites W1973795231 @default.
- W1821047819 cites W1975713674 @default.
- W1821047819 cites W1977656533 @default.
- W1821047819 cites W1984924601 @default.
- W1821047819 cites W1999951470 @default.
- W1821047819 cites W2009969016 @default.
- W1821047819 cites W2026723658 @default.
- W1821047819 cites W2045776382 @default.
- W1821047819 cites W2045841619 @default.
- W1821047819 cites W2055402903 @default.
- W1821047819 cites W2055657684 @default.
- W1821047819 cites W2063406453 @default.
- W1821047819 cites W2069542174 @default.
- W1821047819 cites W2070896094 @default.
- W1821047819 cites W2072394589 @default.
- W1821047819 cites W2073627380 @default.
- W1821047819 cites W2100349081 @default.
- W1821047819 cites W2105036451 @default.
- W1821047819 cites W2111267412 @default.
- W1821047819 cites W2112196989 @default.
- W1821047819 cites W2126301674 @default.
- W1821047819 cites W2128609649 @default.
- W1821047819 cites W2141089321 @default.
- W1821047819 cites W2142267371 @default.
- W1821047819 cites W2168347838 @default.
- W1821047819 cites W2170347395 @default.
- W1821047819 cites W2170895924 @default.
- W1821047819 cites W2177564156 @default.
- W1821047819 doi "https://doi.org/10.1039/c5an00988j" @default.
- W1821047819 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4516631" @default.
- W1821047819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26160546" @default.
- W1821047819 hasPublicationYear "2015" @default.
- W1821047819 type Work @default.
- W1821047819 sameAs 1821047819 @default.
- W1821047819 citedByCount "13" @default.
- W1821047819 countsByYear W18210478192016 @default.
- W1821047819 countsByYear W18210478192017 @default.
- W1821047819 countsByYear W18210478192018 @default.
- W1821047819 countsByYear W18210478192021 @default.
- W1821047819 countsByYear W18210478192023 @default.
- W1821047819 crossrefType "journal-article" @default.
- W1821047819 hasAuthorship W1821047819A5016293970 @default.
- W1821047819 hasAuthorship W1821047819A5023886935 @default.
- W1821047819 hasAuthorship W1821047819A5029703684 @default.
- W1821047819 hasAuthorship W1821047819A5059575527 @default.
- W1821047819 hasAuthorship W1821047819A5069852433 @default.
- W1821047819 hasAuthorship W1821047819A5088362530 @default.
- W1821047819 hasBestOaLocation W18210478192 @default.
- W1821047819 hasConcept C106487976 @default.
- W1821047819 hasConcept C119128265 @default.
- W1821047819 hasConcept C120665830 @default.
- W1821047819 hasConcept C121332964 @default.
- W1821047819 hasConcept C127413603 @default.
- W1821047819 hasConcept C132651336 @default.
- W1821047819 hasConcept C146978453 @default.
- W1821047819 hasConcept C152815550 @default.
- W1821047819 hasConcept C159985019 @default.
- W1821047819 hasConcept C161790260 @default.
- W1821047819 hasConcept C171250308 @default.
- W1821047819 hasConcept C185592680 @default.
- W1821047819 hasConcept C192562407 @default.
- W1821047819 hasConcept C196806460 @default.
- W1821047819 hasConcept C199360897 @default.
- W1821047819 hasConcept C2779843651 @default.
- W1821047819 hasConcept C41008148 @default.
- W1821047819 hasConcept C42067758 @default.
- W1821047819 hasConcept C43617362 @default.
- W1821047819 hasConcept C49040817 @default.
- W1821047819 hasConcept C55493867 @default.
- W1821047819 hasConcept C6648577 @default.
- W1821047819 hasConcept C8673954 @default.
- W1821047819 hasConcept C98015330 @default.
- W1821047819 hasConceptScore W1821047819C106487976 @default.
- W1821047819 hasConceptScore W1821047819C119128265 @default.
- W1821047819 hasConceptScore W1821047819C120665830 @default.
- W1821047819 hasConceptScore W1821047819C121332964 @default.
- W1821047819 hasConceptScore W1821047819C127413603 @default.
- W1821047819 hasConceptScore W1821047819C132651336 @default.
- W1821047819 hasConceptScore W1821047819C146978453 @default.
- W1821047819 hasConceptScore W1821047819C152815550 @default.
- W1821047819 hasConceptScore W1821047819C159985019 @default.
- W1821047819 hasConceptScore W1821047819C161790260 @default.
- W1821047819 hasConceptScore W1821047819C171250308 @default.
- W1821047819 hasConceptScore W1821047819C185592680 @default.