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- W1821574909 abstract "We examined DNA site recognition by Bicoid and its importance for pattern formation in developing Drosophila embryos. Using altered DNA specificity Bicoid mutants and appropriate reporter genes, we show that Bicoid distinguishes among related DNA-binding sites in vivo by a specific contact between amino acid 9 of its recognition alpha-helix (lysine 50 of the homeodomain) and bp 7 of the site. This result is consistent with our earlier results using Saccharomyces cerevisiae but differs from that predicted by crystallographic analysis of another homeodomain-DNA interaction. Our results also demonstrate that Bicoid binds directly to those genes whose transcription it regulates and that the amino acid 9 contact is necessary for Bicoid to direct anterior pattern formation. In both Drosophila embryos and yeast cells, Bicoid requires multiple binding sites to activate transcription of target genes. We find that the distance between binding sites is critical for Bicoid activation but that, unexpectedly, this critical distance differs between Drosophila and S. cerevisiae. This result suggests that Bicoid activation in Drosophila might require an ancillary protein(s) not present in S. cerevisiae." @default.
- W1821574909 created "2016-06-24" @default.
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- W1821574909 creator A5065166765 @default.
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- W1821574909 date "1994-05-01" @default.
- W1821574909 modified "2023-10-11" @default.
- W1821574909 title "Specific DNA recognition and intersite spacing are critical for action of the bicoid morphogen." @default.
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- W1821574909 doi "https://doi.org/10.1128/mcb.14.5.3364" @default.
- W1821574909 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/358702" @default.
- W1821574909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7909358" @default.
- W1821574909 hasPublicationYear "1994" @default.
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