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- W1821604549 abstract "End-group labeling techniques have been proved to be useful analytical tools for the elucidation of primary structures of biological macromolecules. Two methods have been described for the labeling of the 3' ends of deoxyoligonucleotides. In one of the latter, acetylation of the 3' terminal hydroxyl group with radioactive acetic anhydride is used. This chapter describes the recently developed second technique that allows the specific labeling of the 3' terminus of a given deoxyoligonucleotide with a [32P]ribonucleotidyl residue. The addition of 5'-[32P]ribonucleotidyl residues to the 3' end of a deoxyoligonucleotide (I) is catalyzed by the enzyme terminal deoxynucleotidyltransferase with [α-32P]ribonucleoside triphosphates as substrates. The result of this reaction is a mixture of products with one (II) and two (III) nucleotidyl residues added at the 3' end of the primer. The labeling method can be applied for the partial sequence determination of oligodeoxynucleotides." @default.
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- W1821604549 date "1974-01-01" @default.
- W1821604549 modified "2023-10-18" @default.
- W1821604549 title "[26] 3′ End-group labeling and partial sequence determination of oligodeoxynucleotides" @default.
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- W1821604549 doi "https://doi.org/10.1016/0076-6879(74)29030-x" @default.
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