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- W1821824346 abstract "Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CAThe role of long non-coding RNAs (lncRNAs) in various physiological and pathological conditions has been increasingly appreciated, however, given the large number of lncRNAs, little is known for the vast majority of lncRNAs for their potential role in breast cancer. In this study, using real-time PCR-based lncRNA profiling array, we identified a set of lncRNAs that were differentially expressed in breast cancer tissues of patients as compared to the normal breast tissue. Among them, AK023948 was upregulated in breast cancer; it was also upregulated in breast cancer cell lines (MCF-7 and MDA-MB-231) as compared to human mammary epithelial cells (HMLE). This was further supported by breast cancer tissue microarray by in situ hybridization. Moreover, ectopic expression of AK023948 in MCF-7 cells promoted the proliferation and anchorage independent cell growth, implying its potential oncogenic role in breast cancer progression. In consistent with this finding, we found that while ectopic expression of AK023948 activated, AK023948-siRNA suppressed Akt activation. To determine the underlying mechanism of AK023948-mediated activation of Akt, we performed RNA pull-down assay with biotin-labeled AK023948 RNA probe. Mass spectrometry analysis of the pulled down proteins identified ATP dependent RNA helicase A (RHA/DHX-9) as a potential binding partner for AK023948, which was confirmed by western blot. Gene silencing of DHX-9 in MCF-7 cells suppressed AKT phosphorylation, suggesting that AK023948 may activate AKT activation through interacting with DHX-9 and modulating its function. Together, these results suggest that AK023948 may function as an oncogenic lncRNA in breast cancer and thus, it may represent a novel target for breast cancer therapy.Citation Format: Pratirodh Koirala. Long non-coding RNA AK023948 enhances breast cancer progression by activating AKT. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 438. doi:10.1158/1538-7445.AM2014-438" @default.
- W1821824346 created "2016-06-24" @default.
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- W1821824346 date "2014-09-30" @default.
- W1821824346 modified "2023-09-27" @default.
- W1821824346 title "Abstract 438: Long non-coding RNA AK023948 enhances breast cancer progression by activating AKT" @default.
- W1821824346 doi "https://doi.org/10.1158/1538-7445.am2014-438" @default.
- W1821824346 hasPublicationYear "2014" @default.
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